Decomposition of myceliar matrix and extraction of chitosan from Gongronella butleri USDB 0201 and Absidia coeruleareak ATCC 14076

Free chitosan, 2 g/100 g mycelia from Gongronella butleri and 6.5 g/100g mycelia from Absidia coerulea were isolated by 1M NaOH at 45 ?C for 13 h and 0.35M acetic acid at 95 ?C for 5 h. Both myceliar matrixes did not break down under these conditions. However, myceliar matrix could be decomposed by treatment with 11M NaOH at 45 ?C for 13 h and 0.35M acetic acid at 95 ?C for 5 h and then extracted the total chitosan, 8-9 g/100 g mycelia from both fungi. According to these results, G. butleri has higher amount of complexed chitosan and A. coerulea has higher amount in free chitosan.

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Bibliographic Details
Main Authors: Nwe, Nitar, Stevens, Willem, Montet, Didier, Tokura, Seiichi, Tamura, Hiroshi
Format: article biblioteca
Language:eng
Subjects:Q60 - Traitement des produits agricoles non alimentaires, chitosane, Absidia, acide acétique, hydroxyde de sodium, extraction, dégradation, Mucorales, http://aims.fao.org/aos/agrovoc/c_28288, http://aims.fao.org/aos/agrovoc/c_26, http://aims.fao.org/aos/agrovoc/c_80, http://aims.fao.org/aos/agrovoc/c_16040, http://aims.fao.org/aos/agrovoc/c_36910, http://aims.fao.org/aos/agrovoc/c_2159, http://aims.fao.org/aos/agrovoc/c_31668,
Online Access:http://agritrop.cirad.fr/544454/
http://agritrop.cirad.fr/544454/1/document_544454.pdf
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Summary:Free chitosan, 2 g/100 g mycelia from Gongronella butleri and 6.5 g/100g mycelia from Absidia coerulea were isolated by 1M NaOH at 45 ?C for 13 h and 0.35M acetic acid at 95 ?C for 5 h. Both myceliar matrixes did not break down under these conditions. However, myceliar matrix could be decomposed by treatment with 11M NaOH at 45 ?C for 13 h and 0.35M acetic acid at 95 ?C for 5 h and then extracted the total chitosan, 8-9 g/100 g mycelia from both fungi. According to these results, G. butleri has higher amount of complexed chitosan and A. coerulea has higher amount in free chitosan.