Metabolismo de açucares durante o densenvolvimento de frutos de café
In this study, we investigated the sucrose synthase (Susy: EC2.4.1.13) at the biochemical and molecular levels during the development of coffee fruits. Our results suggest that Susy is the main enzyme responsible for sucrose metabolism in coffee fruits. In the pulp and endosperm of fruits at the last stage of maturation, a peak of Susy activity was observed and correlates with an increase of sucrose. At the molecular level, we cloned two cDNAs encoding different Susy isofomus. We also showed that both Susy-encoding genes were expressed in coffee fruits, with differences regarding their spatial and temporal expression. In addition, feeding experiments with 14C-sucrose and incubation of fruits with 14CO2 were carried out. These experiments showed that sugars are not only transported from the leaves to the fruits, but also there is an intense communication among the tissues composing the fruits.
| Main Authors: | , , , , , , , |
|---|---|
| Format: | conference_item biblioteca |
| Language: | por |
| Published: |
EMBRAPA
|
| Subjects: | F62 - Physiologie végétale - Croissance et développement, F60 - Physiologie et biochimie végétale, Coffea arabica, fève de café, développement biologique, fruit (botanique), métabolisme des glucides, expression des gènes, activité enzymatique, ligase, saccharose, Clonage moléculaire, http://aims.fao.org/aos/agrovoc/c_1721, http://aims.fao.org/aos/agrovoc/c_28379, http://aims.fao.org/aos/agrovoc/c_921, http://aims.fao.org/aos/agrovoc/c_3119, http://aims.fao.org/aos/agrovoc/c_1299, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_4320, http://aims.fao.org/aos/agrovoc/c_7495, http://aims.fao.org/aos/agrovoc/c_27503, |
| Online Access: | http://agritrop.cirad.fr/530219/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | In this study, we investigated the sucrose synthase (Susy: EC2.4.1.13) at the biochemical and molecular levels during the development of coffee fruits. Our results suggest that Susy is the main enzyme responsible for sucrose metabolism in coffee fruits. In the pulp and endosperm of fruits at the last stage of maturation, a peak of Susy activity was observed and correlates with an increase of sucrose. At the molecular level, we cloned two cDNAs encoding different Susy isofomus. We also showed that both Susy-encoding genes were expressed in coffee fruits, with differences regarding their spatial and temporal expression. In addition, feeding experiments with 14C-sucrose and incubation of fruits with 14CO2 were carried out. These experiments showed that sugars are not only transported from the leaves to the fruits, but also there is an intense communication among the tissues composing the fruits. |
|---|