Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations
Background: Organophosphate and carbamate insecticides irreversibly inhibit acetylcholinesterase causing death of insects. Resistance-modified acetylcholinesterases (AChEs) have been described in many insect species and sequencing of their genes allowed several point mutations to be described. However, their relative frequency and their cartography had not yet been addressed. Results: To analyze the most frequent mutations providing insecticide resistance in Drosophila melanogaster acetylcholinesterase, the Ace gene was cloned and sequenced in several strains harvested from different parts of the world. Sequence comparison revealed four widespread mutations, II6IV, G265A, F330Y and G368A. We confirm here that mutations are found either isolated or in combination in the same protein and we show that most natural populations are heterogeneous, composed of a mixture of different alleles. In vitro expression of mutated proteins showed that combining mutations in the same protein has two consequences: it increases resistance level and provides a wide spectrum of resistance. Conclusion: The presence of several alleles in natural populations, offering various resistance to carbamate and organophosphate compounds will complicate the establishment of resistance management programs.
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Subjects: | H10 - Ravageurs des plantes, L10 - Génétique et amélioration des animaux, Drosophila melanogaster, acétylcholinestérase, mutation, résistance aux produits chimiques, insecticide, http://aims.fao.org/aos/agrovoc/c_30543, http://aims.fao.org/aos/agrovoc/c_85, http://aims.fao.org/aos/agrovoc/c_5014, http://aims.fao.org/aos/agrovoc/c_5736, http://aims.fao.org/aos/agrovoc/c_3887, |
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dig-cirad-fr-5187942024-01-28T12:12:32Z http://agritrop.cirad.fr/518794/ http://agritrop.cirad.fr/518794/ Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations. Menozzi Philippe, Ming An Shi, Lougarre André, Zhen Hua Tang, Fournier Didier. 2004. BMC Evolutionary Biology, 4https://doi.org/10.1186/1471-2148-4-4 <https://doi.org/10.1186/1471-2148-4-4> Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations Menozzi, Philippe Ming An Shi, Lougarre, André Zhen Hua Tang, Fournier, Didier eng 2004 BMC Evolutionary Biology H10 - Ravageurs des plantes L10 - Génétique et amélioration des animaux Drosophila melanogaster acétylcholinestérase mutation résistance aux produits chimiques insecticide http://aims.fao.org/aos/agrovoc/c_30543 http://aims.fao.org/aos/agrovoc/c_85 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5736 http://aims.fao.org/aos/agrovoc/c_3887 Background: Organophosphate and carbamate insecticides irreversibly inhibit acetylcholinesterase causing death of insects. Resistance-modified acetylcholinesterases (AChEs) have been described in many insect species and sequencing of their genes allowed several point mutations to be described. However, their relative frequency and their cartography had not yet been addressed. Results: To analyze the most frequent mutations providing insecticide resistance in Drosophila melanogaster acetylcholinesterase, the Ace gene was cloned and sequenced in several strains harvested from different parts of the world. Sequence comparison revealed four widespread mutations, II6IV, G265A, F330Y and G368A. We confirm here that mutations are found either isolated or in combination in the same protein and we show that most natural populations are heterogeneous, composed of a mixture of different alleles. In vitro expression of mutated proteins showed that combining mutations in the same protein has two consequences: it increases resistance level and provides a wide spectrum of resistance. Conclusion: The presence of several alleles in natural populations, offering various resistance to carbamate and organophosphate compounds will complicate the establishment of resistance management programs. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/518794/1/518794.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1186/1471-2148-4-4 10.1186/1471-2148-4-4 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=180635 info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2148-4-4 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/1471-2148-4-4 info:eu-repo/semantics/reference/purl/http://www.biomedcentral.com/1471-2148/4/4 |
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H10 - Ravageurs des plantes L10 - Génétique et amélioration des animaux Drosophila melanogaster acétylcholinestérase mutation résistance aux produits chimiques insecticide http://aims.fao.org/aos/agrovoc/c_30543 http://aims.fao.org/aos/agrovoc/c_85 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5736 http://aims.fao.org/aos/agrovoc/c_3887 H10 - Ravageurs des plantes L10 - Génétique et amélioration des animaux Drosophila melanogaster acétylcholinestérase mutation résistance aux produits chimiques insecticide http://aims.fao.org/aos/agrovoc/c_30543 http://aims.fao.org/aos/agrovoc/c_85 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5736 http://aims.fao.org/aos/agrovoc/c_3887 |
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H10 - Ravageurs des plantes L10 - Génétique et amélioration des animaux Drosophila melanogaster acétylcholinestérase mutation résistance aux produits chimiques insecticide http://aims.fao.org/aos/agrovoc/c_30543 http://aims.fao.org/aos/agrovoc/c_85 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5736 http://aims.fao.org/aos/agrovoc/c_3887 H10 - Ravageurs des plantes L10 - Génétique et amélioration des animaux Drosophila melanogaster acétylcholinestérase mutation résistance aux produits chimiques insecticide http://aims.fao.org/aos/agrovoc/c_30543 http://aims.fao.org/aos/agrovoc/c_85 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5736 http://aims.fao.org/aos/agrovoc/c_3887 Menozzi, Philippe Ming An Shi, Lougarre, André Zhen Hua Tang, Fournier, Didier Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations |
description |
Background: Organophosphate and carbamate insecticides irreversibly inhibit acetylcholinesterase causing death of insects. Resistance-modified acetylcholinesterases (AChEs) have been described in many insect species and sequencing of their genes allowed several point mutations to be described. However, their relative frequency and their cartography had not yet been addressed. Results: To analyze the most frequent mutations providing insecticide resistance in Drosophila melanogaster acetylcholinesterase, the Ace gene was cloned and sequenced in several strains harvested from different parts of the world. Sequence comparison revealed four widespread mutations, II6IV, G265A, F330Y and G368A. We confirm here that mutations are found either isolated or in combination in the same protein and we show that most natural populations are heterogeneous, composed of a mixture of different alleles. In vitro expression of mutated proteins showed that combining mutations in the same protein has two consequences: it increases resistance level and provides a wide spectrum of resistance. Conclusion: The presence of several alleles in natural populations, offering various resistance to carbamate and organophosphate compounds will complicate the establishment of resistance management programs. |
format |
article |
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H10 - Ravageurs des plantes L10 - Génétique et amélioration des animaux Drosophila melanogaster acétylcholinestérase mutation résistance aux produits chimiques insecticide http://aims.fao.org/aos/agrovoc/c_30543 http://aims.fao.org/aos/agrovoc/c_85 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5736 http://aims.fao.org/aos/agrovoc/c_3887 |
author |
Menozzi, Philippe Ming An Shi, Lougarre, André Zhen Hua Tang, Fournier, Didier |
author_facet |
Menozzi, Philippe Ming An Shi, Lougarre, André Zhen Hua Tang, Fournier, Didier |
author_sort |
Menozzi, Philippe |
title |
Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations |
title_short |
Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations |
title_full |
Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations |
title_fullStr |
Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations |
title_full_unstemmed |
Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations |
title_sort |
mutations of acetylcholinesterase which confer insecticide resistance in drosophila melanogaster populations |
url |
http://agritrop.cirad.fr/518794/ http://agritrop.cirad.fr/518794/1/518794.pdf |
work_keys_str_mv |
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_version_ |
1792495778230435840 |