![Strategies for identification of genes of agricultural importance in Musa acuminata 'Calcutta 4' [S22-33]](/search/themes/bootstrap3/images/libros.png)
Strategies for identification of genes of agricultural importance in Musa acuminata 'Calcutta 4' [S22-33]
Commercial banana varieties (Musa spp.) are cultivated in approximately 120 countries, generating a global production of around 100 million tons per year. Latin America is responsible for 30% of this production, with Brazil representing the third largest producer world-wide, with a production area of 560 thousand hectares and an annual production of 6 million tons. In 1998, the fungal pathogen Mycosphaerella fijiensis, causal organism of black leaf streak disease, which results in decomposition of leaf area and potential yield losses of up to 50%, was reported for the first time in Brazil in the Amazon region. Over the last 5 years the pathogen has subsequently spread over 6 states in the north of the country. The objectives of this current study are to firstly identify resistance genes in Musa acuminata 'Calcutta 4' via development and characterisation of ESTs from inoculated (M. fljiensis) and un-inoculated leaves, as well as through identification of resistance gene analogs (RGAs) using degenerated primers designed from highly conserved domains (NBS and LRR). A second objective of the study focuses upon examining microsynteny between Musa and Gramineas, specifically through a detailed comparison of sequences of selected M. acuminata 'Calcutta 4' BAC clones with stretches of the rice genome, as a contribution to collaborative studies with other members of the Global Musa Genomics Consortium. To date, specific PCR amplification using degenerative primers targeting NBS and LRR R-gene subfamily domains has generated 12 sequence clusters which, based upon BlastX analysis, show homology to resistance genes and RGAs. In collaboration with CIRAD, 5 BAC clones have been selected, anchored to rice and sorghum genomes with EGRAM (cDNA) markers, subcloned via hydro-shearing, and are currently being sequenced. Three cDNA libraries are also being developed for subsequent 5' single pass sequencing and EST database generation. (Texte intégral)
| Main Authors: | , , , , , , , , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
ISPMB Office
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| Subjects: | F30 - Génétique et amélioration des plantes, Musa acuminata, banque de gènes, gène, résistance aux maladies, Mycosphaerella fijiensis, génie génétique, http://aims.fao.org/aos/agrovoc/c_4994, http://aims.fao.org/aos/agrovoc/c_11116, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_27259, http://aims.fao.org/aos/agrovoc/c_15974, |
| Online Access: | http://agritrop.cirad.fr/514072/ |
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| Summary: | Commercial banana varieties (Musa spp.) are cultivated in approximately 120 countries, generating a global production of around 100 million tons per year. Latin America is responsible for 30% of this production, with Brazil representing the third largest producer world-wide, with a production area of 560 thousand hectares and an annual production of 6 million tons. In 1998, the fungal pathogen Mycosphaerella fijiensis, causal organism of black leaf streak disease, which results in decomposition of leaf area and potential yield losses of up to 50%, was reported for the first time in Brazil in the Amazon region. Over the last 5 years the pathogen has subsequently spread over 6 states in the north of the country. The objectives of this current study are to firstly identify resistance genes in Musa acuminata 'Calcutta 4' via development and characterisation of ESTs from inoculated (M. fljiensis) and un-inoculated leaves, as well as through identification of resistance gene analogs (RGAs) using degenerated primers designed from highly conserved domains (NBS and LRR). A second objective of the study focuses upon examining microsynteny between Musa and Gramineas, specifically through a detailed comparison of sequences of selected M. acuminata 'Calcutta 4' BAC clones with stretches of the rice genome, as a contribution to collaborative studies with other members of the Global Musa Genomics Consortium. To date, specific PCR amplification using degenerative primers targeting NBS and LRR R-gene subfamily domains has generated 12 sequence clusters which, based upon BlastX analysis, show homology to resistance genes and RGAs. In collaboration with CIRAD, 5 BAC clones have been selected, anchored to rice and sorghum genomes with EGRAM (cDNA) markers, subcloned via hydro-shearing, and are currently being sequenced. Three cDNA libraries are also being developed for subsequent 5' single pass sequencing and EST database generation. (Texte intégral) |
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