Lipid-induced pore formation of the Bacillus thuringiensis Cry1Aa insecticidal toxin
After activation, Bacillus thuringiensis (Bt) insecticidal toxin forms pores in larval midgut epithelial cell membranes, leading to host death. Although the crystal structure of the soluble form of Cry1Aa has been determined, the conformation of the pores and the mechanism of toxin interaction with and insertion into membranes are still not clear. Here we show that Cry1Aa spontaneously inserts into lipid mono- and bilayer membranes of appropriate compositions. Fourier Transform InfraRed spectroscopy (FTIR) indicates that insertion is accompanied by conformational changes characterized mainly by an unfolding of the ß-sheet domains. Moreover, Atomic Force Microscopy (AFM) imaging strongly suggests that the pores are composed of four subunits surrounding a 1.5 nm diameter central depression.
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | article biblioteca |
| Language: | eng |
| Subjects: | H10 - Ravageurs des plantes, Bacillus thuringiensis, protéine microbienne, insecticide, pesticide bactérien, membrane cellulaire, structure cellulaire, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_12537, http://aims.fao.org/aos/agrovoc/c_3887, http://aims.fao.org/aos/agrovoc/c_767, http://aims.fao.org/aos/agrovoc/c_9693, http://aims.fao.org/aos/agrovoc/c_1414, |
| Online Access: | http://agritrop.cirad.fr/487157/ http://agritrop.cirad.fr/487157/1/487157.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | After activation, Bacillus thuringiensis (Bt) insecticidal toxin forms pores in larval midgut epithelial cell membranes, leading to host death. Although the crystal structure of the soluble form of Cry1Aa has been determined, the conformation of the pores and the mechanism of toxin interaction with and insertion into membranes are still not clear. Here we show that Cry1Aa spontaneously inserts into lipid mono- and bilayer membranes of appropriate compositions. Fourier Transform InfraRed spectroscopy (FTIR) indicates that insertion is accompanied by conformational changes characterized mainly by an unfolding of the ß-sheet domains. Moreover, Atomic Force Microscopy (AFM) imaging strongly suggests that the pores are composed of four subunits surrounding a 1.5 nm diameter central depression. |
|---|