A method to estimate acetylcholinesterase-active sites and turnover in insects
Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy) -1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific.
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| Main Authors: | , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | H10 - Ravageurs des plantes, insecticide, acétylcholinestérase, propriété des pesticides, résistance aux pesticides, lutte anti-insecte, lutte chimique, Drosophila melanogaster, activité enzymatique, inhibition, http://aims.fao.org/aos/agrovoc/c_3887, http://aims.fao.org/aos/agrovoc/c_85, http://aims.fao.org/aos/agrovoc/c_5732, http://aims.fao.org/aos/agrovoc/c_25427, http://aims.fao.org/aos/agrovoc/c_3885, http://aims.fao.org/aos/agrovoc/c_1514, http://aims.fao.org/aos/agrovoc/c_30543, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_28427, |
| Online Access: | http://agritrop.cirad.fr/476765/ http://agritrop.cirad.fr/476765/1/476765.pdf |
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| Summary: | Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy) -1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific. |
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