Microsatellite markers for genetic population studies in Glossina palpalis gambiensis (Diptera : Glossinidae)

Little is known about intraspeciric variability in tsetse flies and its consequences for vectorial capacity. Microsatellite markers have been developed for Glossina palpalis gambiensis. Three loci have been identified and showed size polymorphisms for insectarium samples. G. palpalis gambiensis from Burkina Faso were also subjected to PCR to investigate their genetic variability. Amplifications were observed in different species belonging to the palpalis group. These molecular markers will be useful to estimate gene flow within G. palpalis gambiensis populations and analysis could be extended to related species.

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Bibliographic Details
Main Authors: Solano, Philippe, Duvallet, Gérard, Dumas, V., Cuisance, Dominique, Cuny, Gérard, Touré, Saydil
Format: article biblioteca
Language:eng
Subjects:L72 - Organismes nuisibles des animaux, L10 - Génétique et amélioration des animaux, Glossina pallidipes, microsatellite, marqueur génétique, génétique des populations, http://aims.fao.org/aos/agrovoc/c_30557, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_34326, http://aims.fao.org/aos/agrovoc/c_8081,
Online Access:http://agritrop.cirad.fr/467979/
http://agritrop.cirad.fr/467979/1/467979.pdf
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Summary:Little is known about intraspeciric variability in tsetse flies and its consequences for vectorial capacity. Microsatellite markers have been developed for Glossina palpalis gambiensis. Three loci have been identified and showed size polymorphisms for insectarium samples. G. palpalis gambiensis from Burkina Faso were also subjected to PCR to investigate their genetic variability. Amplifications were observed in different species belonging to the palpalis group. These molecular markers will be useful to estimate gene flow within G. palpalis gambiensis populations and analysis could be extended to related species.