Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies
Cooperation of two crystal proteins from #Bacillus thuringiensis# subsp. #thompsoni#. strain HnC was shown to be essential for the formation of inclusion bodies. Expression of the operon containing the 34-kDa and 40-kDa protein genes from HnC in a #B. thuringiensis# crystal minus strain resulted in the formation of inclusion bodies identical to those from strain HnC. Interruption of one of the genes in the operon led to the lack of inclusion body and to low production of the remaining protein. Absence of inclusion body and low rate of protein production were also observed when both genes were simultaneously expressed but on different vectors. To show a cooperative effect in the formation of the inclusion body, both proteins must be produced from the same transcript.
Main Authors: | , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, U30 - Méthodes de recherche, Bacillus thuringiensis, expression des gènes, protéine, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_6259, |
Online Access: | http://agritrop.cirad.fr/401071/ http://agritrop.cirad.fr/401071/1/401071.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-401071 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-4010712024-01-28T01:10:12Z http://agritrop.cirad.fr/401071/ http://agritrop.cirad.fr/401071/ Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies. Rang Cécile, Bes Martine, Moar William J., Frutos Roger. 1997. FEBS Letters, 412 : 587-591.https://doi.org/10.1016/S0014-5793(97)00866-1 <https://doi.org/10.1016/S0014-5793(97)00866-1> Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies Rang, Cécile Bes, Martine Moar, William J. Frutos, Roger eng 1997 FEBS Letters H20 - Maladies des plantes U30 - Méthodes de recherche Bacillus thuringiensis expression des gènes protéine http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6259 Cooperation of two crystal proteins from #Bacillus thuringiensis# subsp. #thompsoni#. strain HnC was shown to be essential for the formation of inclusion bodies. Expression of the operon containing the 34-kDa and 40-kDa protein genes from HnC in a #B. thuringiensis# crystal minus strain resulted in the formation of inclusion bodies identical to those from strain HnC. Interruption of one of the genes in the operon led to the lack of inclusion body and to low production of the remaining protein. Absence of inclusion body and low rate of protein production were also observed when both genes were simultaneously expressed but on different vectors. To show a cooperative effect in the formation of the inclusion body, both proteins must be produced from the same transcript. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/401071/1/401071.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/S0014-5793(97)00866-1 10.1016/S0014-5793(97)00866-1 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=98637 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0014-5793(97)00866-1 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/S0014-5793(97)00866-1 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes U30 - Méthodes de recherche Bacillus thuringiensis expression des gènes protéine http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6259 H20 - Maladies des plantes U30 - Méthodes de recherche Bacillus thuringiensis expression des gènes protéine http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6259 |
spellingShingle |
H20 - Maladies des plantes U30 - Méthodes de recherche Bacillus thuringiensis expression des gènes protéine http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6259 H20 - Maladies des plantes U30 - Méthodes de recherche Bacillus thuringiensis expression des gènes protéine http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6259 Rang, Cécile Bes, Martine Moar, William J. Frutos, Roger Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies |
description |
Cooperation of two crystal proteins from #Bacillus thuringiensis# subsp. #thompsoni#. strain HnC was shown to be essential for the formation of inclusion bodies. Expression of the operon containing the 34-kDa and 40-kDa protein genes from HnC in a #B. thuringiensis# crystal minus strain resulted in the formation of inclusion bodies identical to those from strain HnC. Interruption of one of the genes in the operon led to the lack of inclusion body and to low production of the remaining protein. Absence of inclusion body and low rate of protein production were also observed when both genes were simultaneously expressed but on different vectors. To show a cooperative effect in the formation of the inclusion body, both proteins must be produced from the same transcript. |
format |
article |
topic_facet |
H20 - Maladies des plantes U30 - Méthodes de recherche Bacillus thuringiensis expression des gènes protéine http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6259 |
author |
Rang, Cécile Bes, Martine Moar, William J. Frutos, Roger |
author_facet |
Rang, Cécile Bes, Martine Moar, William J. Frutos, Roger |
author_sort |
Rang, Cécile |
title |
Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies |
title_short |
Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies |
title_full |
Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies |
title_fullStr |
Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies |
title_full_unstemmed |
Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies |
title_sort |
simultaneous production of the 34-kda and 40-kda proteins from bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies |
url |
http://agritrop.cirad.fr/401071/ http://agritrop.cirad.fr/401071/1/401071.pdf |
work_keys_str_mv |
AT rangcecile simultaneousproductionofthe34kdaand40kdaproteinsfrombacillusthuringiensissubspthompsoniisrequiredfortheformationofinclusionbodies AT besmartine simultaneousproductionofthe34kdaand40kdaproteinsfrombacillusthuringiensissubspthompsoniisrequiredfortheformationofinclusionbodies AT moarwilliamj simultaneousproductionofthe34kdaand40kdaproteinsfrombacillusthuringiensissubspthompsoniisrequiredfortheformationofinclusionbodies AT frutosroger simultaneousproductionofthe34kdaand40kdaproteinsfrombacillusthuringiensissubspthompsoniisrequiredfortheformationofinclusionbodies |
_version_ |
1792492037721817088 |