The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ
Flavanone 3-hydroxylase is an enzyme acting in the central part of the flavonoid biosynthesis pathway. It is generally encoded by a single gene and seems to have a key position for the regulation in this pathway. These two features make a single #f3h# promoter-gus fusion a suitable tool to study both the #f3h# expression and the regulation of this pathway. We present here the spatial and temporal analysis of the expression of an alfalfa #flavanone# 3-hydroxylase# (#f3h#) promoter-gus fusion introduced into #nicotiana benthamiana#. The #medicago sativa# (alfalfa) #f3h# promoter directed gus expression in flowers, stems, leaves and roots. In flowers, GUS activity was observed in pollen grains, in ovules, in ovary placenta and in the epidermis, medullary parenchyma, trichomes and second cortical cellular layer surrounding the vascular bundles of the peduncle. In stems, GUS activity was detected at the same places as in the peduncle except for the medullary parenchyma. In roots, we found GUS staining in root hairs, epidermis and in the vascular bundles of the elongated zone. Finally, in leaves, the #f3h# promoter expressed essentially in the stalk cells of the multicellular trichomes. The expression pattern of the #f3h-gus# fusion was correlated to the presence of flavonoids #in situ#. These data indicate that this construct can be very useful to study factors controlling the production of flavonoids.
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Subjects: | F30 - Génétique et amélioration des plantes, flavonoïde, mesure (activité), fusion de gènes, Nicotiana, expression des gènes, enzyme, fleur, tige, feuille, racine, http://aims.fao.org/aos/agrovoc/c_2964, http://aims.fao.org/aos/agrovoc/c_4668, http://aims.fao.org/aos/agrovoc/c_27528, http://aims.fao.org/aos/agrovoc/c_5175, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_2603, http://aims.fao.org/aos/agrovoc/c_2993, http://aims.fao.org/aos/agrovoc/c_7390, http://aims.fao.org/aos/agrovoc/c_4243, http://aims.fao.org/aos/agrovoc/c_6651, |
Online Access: | http://agritrop.cirad.fr/390004/ http://agritrop.cirad.fr/390004/1/Charrier1996_Article_TheExpressionPatternOfAlfalfaF.pdf |
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dig-cirad-fr-3900042024-01-27T23:11:05Z http://agritrop.cirad.fr/390004/ http://agritrop.cirad.fr/390004/ The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ. Charrier Bénédicte, Le Roux Christine, Kondorosi Adam, Ratet Pascal. 1996. Plant Molecular Biology, 30 : 1153-1168.https://doi.org/10.1007/BF00019549 <https://doi.org/10.1007/BF00019549> The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ Charrier, Bénédicte Le Roux, Christine Kondorosi, Adam Ratet, Pascal eng 1996 Plant Molecular Biology F30 - Génétique et amélioration des plantes flavonoïde mesure (activité) fusion de gènes Nicotiana expression des gènes enzyme fleur tige feuille racine http://aims.fao.org/aos/agrovoc/c_2964 http://aims.fao.org/aos/agrovoc/c_4668 http://aims.fao.org/aos/agrovoc/c_27528 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_2993 http://aims.fao.org/aos/agrovoc/c_7390 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_6651 Flavanone 3-hydroxylase is an enzyme acting in the central part of the flavonoid biosynthesis pathway. It is generally encoded by a single gene and seems to have a key position for the regulation in this pathway. These two features make a single #f3h# promoter-gus fusion a suitable tool to study both the #f3h# expression and the regulation of this pathway. We present here the spatial and temporal analysis of the expression of an alfalfa #flavanone# 3-hydroxylase# (#f3h#) promoter-gus fusion introduced into #nicotiana benthamiana#. The #medicago sativa# (alfalfa) #f3h# promoter directed gus expression in flowers, stems, leaves and roots. In flowers, GUS activity was observed in pollen grains, in ovules, in ovary placenta and in the epidermis, medullary parenchyma, trichomes and second cortical cellular layer surrounding the vascular bundles of the peduncle. In stems, GUS activity was detected at the same places as in the peduncle except for the medullary parenchyma. In roots, we found GUS staining in root hairs, epidermis and in the vascular bundles of the elongated zone. Finally, in leaves, the #f3h# promoter expressed essentially in the stalk cells of the multicellular trichomes. The expression pattern of the #f3h-gus# fusion was correlated to the presence of flavonoids #in situ#. These data indicate that this construct can be very useful to study factors controlling the production of flavonoids. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/390004/1/Charrier1996_Article_TheExpressionPatternOfAlfalfaF.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/BF00019549 10.1007/BF00019549 info:eu-repo/semantics/altIdentifier/doi/10.1007/BF00019549 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/BF00019549 |
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F30 - Génétique et amélioration des plantes flavonoïde mesure (activité) fusion de gènes Nicotiana expression des gènes enzyme fleur tige feuille racine http://aims.fao.org/aos/agrovoc/c_2964 http://aims.fao.org/aos/agrovoc/c_4668 http://aims.fao.org/aos/agrovoc/c_27528 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_2993 http://aims.fao.org/aos/agrovoc/c_7390 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_6651 F30 - Génétique et amélioration des plantes flavonoïde mesure (activité) fusion de gènes Nicotiana expression des gènes enzyme fleur tige feuille racine http://aims.fao.org/aos/agrovoc/c_2964 http://aims.fao.org/aos/agrovoc/c_4668 http://aims.fao.org/aos/agrovoc/c_27528 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_2993 http://aims.fao.org/aos/agrovoc/c_7390 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_6651 |
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F30 - Génétique et amélioration des plantes flavonoïde mesure (activité) fusion de gènes Nicotiana expression des gènes enzyme fleur tige feuille racine http://aims.fao.org/aos/agrovoc/c_2964 http://aims.fao.org/aos/agrovoc/c_4668 http://aims.fao.org/aos/agrovoc/c_27528 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_2993 http://aims.fao.org/aos/agrovoc/c_7390 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_6651 F30 - Génétique et amélioration des plantes flavonoïde mesure (activité) fusion de gènes Nicotiana expression des gènes enzyme fleur tige feuille racine http://aims.fao.org/aos/agrovoc/c_2964 http://aims.fao.org/aos/agrovoc/c_4668 http://aims.fao.org/aos/agrovoc/c_27528 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_2993 http://aims.fao.org/aos/agrovoc/c_7390 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_6651 Charrier, Bénédicte Le Roux, Christine Kondorosi, Adam Ratet, Pascal The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ |
description |
Flavanone 3-hydroxylase is an enzyme acting in the central part of the flavonoid biosynthesis pathway. It is generally encoded by a single gene and seems to have a key position for the regulation in this pathway. These two features make a single #f3h# promoter-gus fusion a suitable tool to study both the #f3h# expression and the regulation of this pathway. We present here the spatial and temporal analysis of the expression of an alfalfa #flavanone# 3-hydroxylase# (#f3h#) promoter-gus fusion introduced into #nicotiana benthamiana#. The #medicago sativa# (alfalfa) #f3h# promoter directed gus expression in flowers, stems, leaves and roots. In flowers, GUS activity was observed in pollen grains, in ovules, in ovary placenta and in the epidermis, medullary parenchyma, trichomes and second cortical cellular layer surrounding the vascular bundles of the peduncle. In stems, GUS activity was detected at the same places as in the peduncle except for the medullary parenchyma. In roots, we found GUS staining in root hairs, epidermis and in the vascular bundles of the elongated zone. Finally, in leaves, the #f3h# promoter expressed essentially in the stalk cells of the multicellular trichomes. The expression pattern of the #f3h-gus# fusion was correlated to the presence of flavonoids #in situ#. These data indicate that this construct can be very useful to study factors controlling the production of flavonoids. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes flavonoïde mesure (activité) fusion de gènes Nicotiana expression des gènes enzyme fleur tige feuille racine http://aims.fao.org/aos/agrovoc/c_2964 http://aims.fao.org/aos/agrovoc/c_4668 http://aims.fao.org/aos/agrovoc/c_27528 http://aims.fao.org/aos/agrovoc/c_5175 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_2993 http://aims.fao.org/aos/agrovoc/c_7390 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_6651 |
author |
Charrier, Bénédicte Le Roux, Christine Kondorosi, Adam Ratet, Pascal |
author_facet |
Charrier, Bénédicte Le Roux, Christine Kondorosi, Adam Ratet, Pascal |
author_sort |
Charrier, Bénédicte |
title |
The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ |
title_short |
The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ |
title_full |
The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ |
title_fullStr |
The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ |
title_full_unstemmed |
The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ |
title_sort |
expression pattern of alfalfa flavanone 3-hydroxylase promoter-gus fusion in nicotiana benthamiana correlates with the presence of flavonoids detected in situ |
url |
http://agritrop.cirad.fr/390004/ http://agritrop.cirad.fr/390004/1/Charrier1996_Article_TheExpressionPatternOfAlfalfaF.pdf |
work_keys_str_mv |
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