Expressão gênica da proteína receptora da rianodina e qualidade da carne em tilápia-do-Nilo submetida ao estresse antes do abate
Fish management procedures in aquiculture, such as capture, handling and transport, are often traumatic and cause serious physiological and biochemical reactions. The importance of the reduction of pre-slaughter stress is due to the fact that vigorous swimming intensely employs the white muscle, increases the anaerobic glycolysis and the production of lactic acid, and decreases muscle´s pH with the structural degradation of muscular proteins. The above diminishes the meat´s water-holding capacity (WHC). The genetic factors bonded to changes in the muscle´s WHC are related to alterations in the activity of ryanodine receptors which may decrease the ability of this channel to control the release of calcium to the cytoplasm of the muscular cell, especially in periods of physical stress. Calcium excess in the cytosol causes fast muscular contraction, with an acceleration of anaerobic metabolism and post-mortem glycolysis. The muscle´s pH is drastically decreased, affecting the muscle´s capacity to retain water in the intracellular stores. Changes in muscle quality derived from pre-slaughter stress diminish shelf life and bring economic liabilities for the fish industry. Current analysis assesses the pre-slaughter stress caused by transport and its influence on stress indicators, fillets´ quality and sensorial factors, and genetic expression of the ryanodine receptor protein (RyR) in Nile tilapias.
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| Format: | Thesis/Dissertation biblioteca |
| Language: | Portuguese |
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Universidade Estadual de Maringá. Centro de Ciências Agrárias. Programa de Pós-Graduação em Zootecnia
2015
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| Subjects: | Cortisol, Colorimetria, Brasil, Hipermetabolismo, Colorimetry, Hypermetabolism, Brazil, Oreochromis niloticus PH, Rianodina, Qualidade da carne, Expressão gênica zx, Receptores, Tilápia-do-Nilo (Oreochromis niloticus), Capacidade de retenção de água, Estresse, Aquicultura, Variedade TILAMAX, Water-holding capacity, |
| Online Access: | http://hdl.handle.net/1834/9824 |
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| Summary: | Fish management procedures in aquiculture, such as capture, handling and transport, are often traumatic and cause serious physiological and biochemical reactions. The importance of the reduction of pre-slaughter stress is due to the fact that vigorous swimming intensely employs the white muscle, increases the anaerobic glycolysis and the production of lactic acid, and decreases muscle´s pH with the structural degradation of muscular proteins. The above diminishes the meat´s water-holding capacity (WHC). The genetic factors bonded to changes in the muscle´s WHC are related to alterations in the activity of ryanodine receptors which may decrease the ability of this channel to control the release of calcium to the cytoplasm of the muscular cell, especially in periods of physical stress. Calcium excess in the cytosol causes fast muscular contraction, with an acceleration of anaerobic metabolism and post-mortem glycolysis. The muscle´s pH is drastically decreased, affecting the muscle´s capacity to retain water in the intracellular stores. Changes in muscle quality derived from pre-slaughter stress diminish shelf life and bring economic liabilities for the fish industry. Current analysis assesses the pre-slaughter stress caused by transport and its influence on stress indicators, fillets´ quality and sensorial factors, and genetic expression of the ryanodine receptor protein (RyR) in Nile tilapias. |
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