Effect of different doses of pituitary hormone on the reproductive performance of shemaya females (Alburnus leobergi, Freyhof & Kottelat, 2007)
The stage of obtaining high-quality gonads was explored in the course of development of the biotechnology for artificial reproduction of the anadromous Azov-Black Sea shemaya Alburnus leobergi (Freyhof & Kottelat, 2007), a valuable species of the Azov Sea ichthyofauna. This research is aimed at the assessment of the maturity status of shemaya females and males after their wintering in the ponds of a fish farm on the Lower Don at a thermal constant of 849 degree days (DD) and at the evaluation of the reproductive parameters of shemaya females exposed to different doses of the pituitary extract. In all literary sources available, the information on this topic has been scarce and fragmentary, and in the foreign sources, it was entirely absent. Our investigation has shown that the gonads of 55 % of males were at 5th and 5th–6th stages of maturity. At the same time, females significantly differed by the degree of gonad maturity: 26.0 % of females had first-generation eggs at different stages of resorption, the sexual products of 15.8 % of females were at the 5th stage of maturity, and 58.2 % of females had immature sexual products at the 3rd–4th and 4th stages of maturity. The investigation of the effect of two doses (6.0 and 9.0 mg/kg) of the pituitary gland extract with application of the fractional method showed that 15.0 % and 16.7 % of injected shemaya females with the most mature eggs responded to the preliminary dose of the pituitary extract by ovulation of physiologically sound eggs (0.6 and 0.9 mg/kg, respectively). 20.0 % and 6.7 % of females responded to the provoking dose of pituitary hormone by ovulating physiologically high-quality eggs in accordance with the concentration applied. The fertilization rate of physiologically sound eggs in both scenarios had similar values (97.4 and 97.9 %). Since the thermal constant of 849 DD is excessive for the start of artificial reproduction of the Azov-Black Sea shemaya, it is necessary to conduct research in order to identify the thermal constant precluding the loss of the first-generation eggs caused by the incipient processes of resorption.
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| Format: | Journal Contribution biblioteca |
| Language: | Russian |
| Published: |
2022
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| Subjects: | Alburnus leobergi, Hormonal stimulation, Gonadosomatic index (GSI), Stages of maturity, Artificial reproduction, Pituitary gland extract, Черноморско-азовская шемая, Гормональная стимуляция, Гонадосоматический индекс (ГСИ), Искусственное воспроизводство, ООО «Рыбколхоз им. Мирошниченко», ASFA_2015::H::Hormones, ASFA_2015::O::Oocytes, ASFA_2015::S::Sexual maturity, |
| Online Access: | http://hdl.handle.net/1834/41953 |
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| Summary: | The stage of obtaining high-quality gonads was explored in the course of development of the biotechnology for artificial reproduction of the anadromous Azov-Black Sea shemaya Alburnus leobergi (Freyhof & Kottelat, 2007), a valuable species of the Azov Sea ichthyofauna. This research is aimed at the assessment of the maturity status of shemaya females and males after their wintering in the ponds of a fish farm on the Lower Don at a thermal constant of 849 degree days (DD) and at the evaluation of the reproductive parameters of shemaya females exposed to different doses of the pituitary extract. In all literary sources available, the information on this topic has been scarce and fragmentary, and in the foreign sources, it was entirely absent. Our investigation has shown that the gonads of 55 % of males were at 5th and 5th–6th stages of maturity. At the same time, females significantly differed by the degree of gonad maturity: 26.0 % of females had first-generation eggs at different stages of resorption, the sexual products of 15.8 % of females were at the 5th stage of maturity, and 58.2 % of females had immature sexual products at the 3rd–4th and 4th stages of maturity. The investigation of the effect of two doses (6.0 and 9.0 mg/kg) of the pituitary gland extract with application of the fractional method showed that 15.0 % and 16.7 % of injected shemaya females with the most mature eggs responded to the preliminary dose of the pituitary extract by ovulation of physiologically sound eggs (0.6 and 0.9 mg/kg, respectively). 20.0 % and 6.7 % of females responded to the provoking dose of pituitary hormone by ovulating physiologically high-quality eggs in accordance with the concentration applied. The fertilization rate of physiologically sound eggs in both scenarios had similar values (97.4 and 97.9 %). Since the thermal constant of 849 DD is excessive for the start of artificial reproduction of the Azov-Black Sea shemaya, it is necessary to conduct research in order to identify the thermal constant precluding the loss of the first-generation eggs caused by the incipient processes of resorption. |
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