Effect of different levels of dietary nucleotide on growth and haemolymph biochemical parameters of female Litopenaeus vannamei (Boone, 1931) during maturation and Eyestalk Ablation

In this study, the effect of different dietary nucleotide levels (0, 0.2, 0.4 and 0.6%) was investigated on growth indices and some haemolymph biochemical parameters of Litopenaeus vannamei female broodstock during 30 days. Broodstocks were fed twice with fresh food and twice with concentrate feed). After 3 weeks of feeding, eyestalk of broodstocks was ablated with hot scissors. Hemolymph was sampled for glucose, total cholesterol, triglyceride, calcium, albumin, total soluble proteins, and high density lipoprotein on the first day of the trial, 21 days (before eyestalk ablation) and 30 days (9 days after eyestalk ablation) after starting the feeding trial. Water parameters including temperature, dissolve oxygen, salinity and pH recorded daily as 27.79±1.24˚C, 5.78±0.41 ppm, 31.82±3.1 ppt and 7.9±0.18, respectively. Weight gain, specific growth rate and daily growth rate in samples treated by dietary nucleotide showed no significant difference (p>0.05) compared to control group. Significant increase (p<0.05) in glucose, total cholesterol, triglyceride, albumin and total protein were observed in all groups compared to control group. In conclusion 0.4 % nucleotide can improve the dietary sexual maturation of Litopenaeus vannamei.

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Bibliographic Details
Main Authors: Arshadi, A., Yavari, V., Oujifard, A., Mousavi, S.M.
Format: article biblioteca
Language:Persian
Published: 2016
Subjects:Biology, Iran, Litopenaeus vannamei, Nucleotide, Heamolymph biochemical parameters, Growth, Maturation, Diet,
Online Access:http://hdl.handle.net/1834/39401
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Summary:In this study, the effect of different dietary nucleotide levels (0, 0.2, 0.4 and 0.6%) was investigated on growth indices and some haemolymph biochemical parameters of Litopenaeus vannamei female broodstock during 30 days. Broodstocks were fed twice with fresh food and twice with concentrate feed). After 3 weeks of feeding, eyestalk of broodstocks was ablated with hot scissors. Hemolymph was sampled for glucose, total cholesterol, triglyceride, calcium, albumin, total soluble proteins, and high density lipoprotein on the first day of the trial, 21 days (before eyestalk ablation) and 30 days (9 days after eyestalk ablation) after starting the feeding trial. Water parameters including temperature, dissolve oxygen, salinity and pH recorded daily as 27.79±1.24˚C, 5.78±0.41 ppm, 31.82±3.1 ppt and 7.9±0.18, respectively. Weight gain, specific growth rate and daily growth rate in samples treated by dietary nucleotide showed no significant difference (p>0.05) compared to control group. Significant increase (p<0.05) in glucose, total cholesterol, triglyceride, albumin and total protein were observed in all groups compared to control group. In conclusion 0.4 % nucleotide can improve the dietary sexual maturation of Litopenaeus vannamei.