Quantification of vitamin C (ascorbic acid) requirements of Heterobranchus longifilis fingerlings
A feeding experiment was conducted to quantify the minimum dietary vitamin C requirement of Heterobranchus longifilis fingerlings and the minimal dietary inclusion levels that will meet these requirements after degradation from feed processing and storage. Fish were fed a basal diet with 42.5% crude protein for a conditioning period of 2 weeks. Following conditioning, fingerlings with initial mean weight, 2.3~c0.3 g were stocked as groups of 20 fingerlings into 30 litre tanks in a mini flowthrough experimental system and fed the basal diet and experimental diets supplemented with 0, 50, 100, 150, 200, or 250 mg of L-ascorbic acid kg-1 diet obtained from TUYIL PHARM. INDUSTRIES, lIorin, included into the basal diet by replacing part of the silica component and fed to triplicate groups for 20 weeks. Fish fed the control (0 mg vitamin C kg-1) diet exhibited deficiency signs including lordosis, caudal fin deformity, skin erosion and significantly suppressed weight gain and higher condition factor. Protein efficiency ratio and specific growth rate were significantly improved with increasing levels of vitamin C up to 200mg kg-1 diet. Tissue (liver, kidney, gills and muscle) ascorbate concentration generally reflected dietary inclusion levels with the signiticant lowest level occurring in the control groups. Vitamin C analysis after feed processing revealed 18 - 21% loss. The least mean squares error regression analysis of weight gain data on inclusion level of vitamin C revealed that the minimum dietary requirement of H. longifilis is 82.2~c0.2 mg vitamin C kg-1 diet which corresponds to 100 mg of vitamin C kg-1 diet based on data from this study.
| Main Authors: | , , , , |
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| Format: | conference_item biblioteca |
| Language: | English |
| Published: |
Fisheries Society of Nigeria
2007
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| Subjects: | Aquaculture, Heterobranchus longifilis, Nigeria, New Bussa, freshwater environment, Fish culture, Feeding experiments, Vitamin C, Growth rate, |
| Online Access: | http://hdl.handle.net/1834/37778 |
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| Summary: | A feeding experiment was conducted to quantify the minimum dietary vitamin C requirement of Heterobranchus longifilis fingerlings and the minimal dietary inclusion levels that will meet these requirements after degradation from feed processing and storage. Fish were fed a basal diet with 42.5% crude protein for a conditioning period of 2 weeks. Following conditioning, fingerlings with initial mean weight, 2.3~c0.3 g were stocked as groups of 20 fingerlings into 30 litre tanks in a mini flowthrough experimental system and fed the basal diet and experimental diets supplemented with 0, 50, 100, 150, 200, or 250 mg of L-ascorbic acid kg-1 diet obtained from TUYIL PHARM. INDUSTRIES, lIorin, included into the basal diet by replacing part of the silica component and fed to triplicate groups for 20 weeks. Fish fed the control (0 mg vitamin C kg-1) diet exhibited deficiency signs including lordosis, caudal fin deformity, skin erosion and significantly suppressed weight gain and higher condition factor. Protein efficiency ratio and specific growth rate were significantly improved with increasing levels of vitamin C up to 200mg kg-1 diet. Tissue (liver, kidney, gills and muscle) ascorbate concentration generally reflected dietary inclusion levels with the signiticant lowest level occurring in the control groups. Vitamin C analysis after feed processing revealed 18 - 21% loss. The least mean squares error regression analysis of weight gain data on inclusion level of vitamin C revealed that the minimum dietary requirement of H. longifilis is 82.2~c0.2 mg vitamin C kg-1 diet which corresponds to 100 mg of vitamin C kg-1 diet based on data from this study. |
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