A Study on population genetic of Hilsa shad, Tenualosa ilisha, in Khouzestan, Iran using molecular method (RAPD)
The genetic structure of Hilsa Shad Tenualosa ilisha in Khouzestan waters including Karoon, Arvandrood and Bahmanshir Rivers as well as Persian Gulf was studied using RAPD technique. After optimizing PCR condition, nine RAPD primers were selected from which 58 polymorphic loci were obtained on 12 specimens from each geographical region (A total of 48 specimens). RAPDPLOT, RAPDDIST and POPGENE computer software were used to analyze the RAPD data. Canonical discriminant analysis was deployed for statistical assessment of the RAPD data. Maximum and minimum genetic distances were found between samples from Arvandrood River and Persian Gulf (0.1987) and Arvandrood and Bahmanshir Rivers (0.0852), respectively. The UPGMA dendrogram showed that the samples from Karoon River and Persian Gulf form one group and samples from Arvandrood and Bahmanshir Rivers form another suggesting the hypothesis that there are Iranian and Iraqi populations of the species that chose their own specific rivers for spawning. According to this hypothesis, the specimens from Persian Gulf chose Karoon as their spawning river. Other populations migrate to Tigris and Euphrates Rivers in Iraq.
Main Authors: | , , , |
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Format: | Journal Contribution biblioteca |
Language: | Persian |
Published: |
2008
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Subjects: | Polymerase chain reaction, Primers, Phylogenetics, Tenualosa ilisha, Population genetics, Marine, |
Online Access: | http://hdl.handle.net/1834/11371 |
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Summary: | The genetic structure of Hilsa Shad Tenualosa ilisha in Khouzestan waters including Karoon, Arvandrood and Bahmanshir Rivers as well as Persian Gulf was studied using RAPD technique. After optimizing PCR condition, nine RAPD primers were selected from which 58 polymorphic loci were obtained on 12 specimens from each geographical region (A total of 48 specimens). RAPDPLOT, RAPDDIST and POPGENE computer software were used to analyze the RAPD data. Canonical discriminant analysis was deployed for statistical assessment of the RAPD data. Maximum and minimum genetic distances were found between samples from Arvandrood River and Persian Gulf (0.1987) and Arvandrood and Bahmanshir Rivers (0.0852), respectively. The UPGMA dendrogram showed that the samples from Karoon River and Persian Gulf form one group and samples from Arvandrood and Bahmanshir Rivers form another suggesting the hypothesis that there are Iranian and Iraqi populations of the species that chose their own specific rivers for spawning. According to this hypothesis, the specimens from Persian Gulf chose Karoon as their spawning river. Other populations migrate to Tigris and Euphrates Rivers in Iraq. |
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