Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta).
Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)). The present work reports the effect of different pH and sucrose concentrations on in vitro propagation of Nephrolepis biserrata. Fronds aseptically obtained from stolon segment culture were cultivated in MS semi-solid medium supplemented with 0, 15, 30, 45 and 60 g.L -1 sucrose and pH adjusted to 3, 5, 7 and 9, in a factorial design. Frond number and length, pinnae number, raquis length and diam eter, fresh and dry matter weight were measured. Inhibition of shoot and leaf regeneration was observed in all the pH treatments in the abs ence of sucrose. On the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at pH 5 and 7, respectively. Sucrose concentrations from 15 to 45g.L -1 increased leaf length and diameter and the pH 9 did not affect the dry matter weight, and was still not adequate for development of new fronds. Young sporophytes were successfully acclimated.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2004-09-07
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Subjects: | Pteridófitas, Cultivo in vitro., Cultura de Tecido, Planta Ornamental., Ornamental plants, Tissue culture, Nephrolepis biserrata., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/154317 |
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dig-alice-doc-1543172017-08-16T03:38:41Z Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta). AMBROSIO, S. T. MELO, N. F. de NATONIEL FRANKLIN DE MELO, CPATSA. Pteridófitas Cultivo in vitro. Cultura de Tecido Planta Ornamental. Ornamental plants Tissue culture Nephrolepis biserrata. Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)). The present work reports the effect of different pH and sucrose concentrations on in vitro propagation of Nephrolepis biserrata. Fronds aseptically obtained from stolon segment culture were cultivated in MS semi-solid medium supplemented with 0, 15, 30, 45 and 60 g.L -1 sucrose and pH adjusted to 3, 5, 7 and 9, in a factorial design. Frond number and length, pinnae number, raquis length and diam eter, fresh and dry matter weight were measured. Inhibition of shoot and leaf regeneration was observed in all the pH treatments in the abs ence of sucrose. On the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at pH 5 and 7, respectively. Sucrose concentrations from 15 to 45g.L -1 increased leaf length and diameter and the pH 9 did not affect the dry matter weight, and was still not adequate for development of new fronds. Young sporophytes were successfully acclimated. 2017-08-04T23:46:24Z 2017-08-04T23:46:24Z 2004-09-07 2004 2018-09-11T11:11:11Z Artigo de periódico Acta Botanica Brasilica, São Paulo, v. 18, n. 4, p. 809-813, 2004. http://www.alice.cnptia.embrapa.br/alice/handle/doc/154317 en eng openAccess |
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Pteridófitas Cultivo in vitro. Cultura de Tecido Planta Ornamental. Ornamental plants Tissue culture Nephrolepis biserrata. Pteridófitas Cultivo in vitro. Cultura de Tecido Planta Ornamental. Ornamental plants Tissue culture Nephrolepis biserrata. |
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Pteridófitas Cultivo in vitro. Cultura de Tecido Planta Ornamental. Ornamental plants Tissue culture Nephrolepis biserrata. Pteridófitas Cultivo in vitro. Cultura de Tecido Planta Ornamental. Ornamental plants Tissue culture Nephrolepis biserrata. AMBROSIO, S. T. MELO, N. F. de Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta). |
description |
Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)). The present work reports the effect of different pH and sucrose concentrations on in vitro propagation of Nephrolepis biserrata. Fronds aseptically obtained from stolon segment culture were cultivated in MS semi-solid medium supplemented with 0, 15, 30, 45 and 60 g.L -1 sucrose and pH adjusted to 3, 5, 7 and 9, in a factorial design. Frond number and length, pinnae number, raquis length and diam eter, fresh and dry matter weight were measured. Inhibition of shoot and leaf regeneration was observed in all the pH treatments in the abs ence of sucrose. On the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at pH 5 and 7, respectively. Sucrose concentrations from 15 to 45g.L -1 increased leaf length and diameter and the pH 9 did not affect the dry matter weight, and was still not adequate for development of new fronds. Young sporophytes were successfully acclimated. |
author2 |
NATONIEL FRANKLIN DE MELO, CPATSA. |
author_facet |
NATONIEL FRANKLIN DE MELO, CPATSA. AMBROSIO, S. T. MELO, N. F. de |
format |
Artigo de periódico |
topic_facet |
Pteridófitas Cultivo in vitro. Cultura de Tecido Planta Ornamental. Ornamental plants Tissue culture Nephrolepis biserrata. |
author |
AMBROSIO, S. T. MELO, N. F. de |
author_sort |
AMBROSIO, S. T. |
title |
Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta). |
title_short |
Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta). |
title_full |
Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta). |
title_fullStr |
Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta). |
title_full_unstemmed |
Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta). |
title_sort |
interaction between sucrose and ph during in vitro culture of nephrolepis biserrata (sw.) schott (pteridophyta). |
publishDate |
2004-09-07 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/154317 |
work_keys_str_mv |
AT ambrosiost interactionbetweensucroseandphduringinvitrocultureofnephrolepisbiserrataswschottpteridophyta AT melonfde interactionbetweensucroseandphduringinvitrocultureofnephrolepisbiserrataswschottpteridophyta |
_version_ |
1756023655029014528 |