Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Separatas biblioteca |
| Language: | English eng |
| Published: |
2017-11-28
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| Subjects: | Callogenesis, Growlh curve., secondary metabolites., |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950 |
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| Summary: | Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day. |
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