Genome Resources of Fusarium oxysporum f. sp. vasinfectum race 1, 4, 5 and 8 that causes wilt diseases in cotton

Fungi in the genus Fusarium are hyphomycetes that are widely distributed in soils and that are often associated with plants and plant diseases. Fusarium oxysporum f.sp. vasinfectum (FOV) was first recognized in 1892, in sandy acid soils in Alabama (USA) and causing a disease called Fusarium wilt of cotton. FOV includes several pathogenic races and has been separated into 4 distinct phylogenetic subgroups based in DNA sequences of a few housekeeping genes. We have developed WGS for isolates of FOV races 1, 4, 5 and 8 using Nanopore sequencing (MinION) and Illumina (Mi-Seq) technology and assembled the contiguous contigs for individual isolates of race 1 (52 Mb), race 4 (68 Mb), race 5 (68 Mb) and race 8 (55 Mb), each FOV race draft contiguous contigs polished with high quality Illumina reads over 25 million and over 99% estimated genome completeness. These genomes will provide a necessary resource for comparative genomics to identify genes or genome features that are involved in pathogenicity, and in different regulatory and diagnostics applications.

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Bibliographic Details
Main Author: Contact; Kurt. A. Zeller, USDA-APHIS-PPQ-S&T (18796819)
Format: Dataset biblioteca
Published: 2020
Subjects:Genetics, Fusarium oxysporum f. sp. vasinfectum, eEukaryotes, genome sequencing,
Online Access:https://figshare.com/articles/dataset/Genome_Resources_of_Fusarium_oxysporum_f_sp_vasinfectum_race_1_4_5_and_8_that_causes_wilt_diseases_in_cotton/25155395
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Description
Summary:Fungi in the genus Fusarium are hyphomycetes that are widely distributed in soils and that are often associated with plants and plant diseases. Fusarium oxysporum f.sp. vasinfectum (FOV) was first recognized in 1892, in sandy acid soils in Alabama (USA) and causing a disease called Fusarium wilt of cotton. FOV includes several pathogenic races and has been separated into 4 distinct phylogenetic subgroups based in DNA sequences of a few housekeeping genes. We have developed WGS for isolates of FOV races 1, 4, 5 and 8 using Nanopore sequencing (MinION) and Illumina (Mi-Seq) technology and assembled the contiguous contigs for individual isolates of race 1 (52 Mb), race 4 (68 Mb), race 5 (68 Mb) and race 8 (55 Mb), each FOV race draft contiguous contigs polished with high quality Illumina reads over 25 million and over 99% estimated genome completeness. These genomes will provide a necessary resource for comparative genomics to identify genes or genome features that are involved in pathogenicity, and in different regulatory and diagnostics applications.