Characterization of NgAgo cleavage sites
NgAgo and mutants were incubated with plasmid DNA and 5'P guide sequences targeting the plasmid. Nicked/cleaved products were amplified via primer extension, which terminates at the nick site, before being used to generate dsDNA that was ligated into pGEM-T-Easy. The library of ligated products were then sequenced. Raw reads from products generated via primers hybridizing on the 5' and 3' end of the target are deposited here.
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| Main Author: | |
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| Format: | Dataset biblioteca |
| Published: |
2021
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| Subjects: | Genetics, raw sequence reads, |
| Online Access: | https://figshare.com/articles/dataset/Characterization_of_NgAgo_cleavage_sites/25088783 |
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| Summary: | NgAgo and mutants were incubated with plasmid DNA and 5'P guide sequences targeting the plasmid. Nicked/cleaved products were amplified via primer extension, which terminates at the nick site, before being used to generate dsDNA that was ligated into pGEM-T-Easy. The library of ligated products were then sequenced. Raw reads from products generated via primers hybridizing on the 5' and 3' end of the target are deposited here. |
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