The beef cattle rumen microbiome composition is influenced by host genetics

The beef cattle rumen microbiome composition is influenced by host genetics

DNA was extracted from the rumen samples using the PowerMag Soil DNA isolation kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol with a few modifications as described by publication. The modified protocol also included adding RNase A to the lysis solution to ensure removal of RNA during DNA extraction. The isolated DNA was stored at -20°C until used for bacterial community analysis. The V4 region of the 16S rRNA gene was amplified and sequenced on the MiSeq platform.

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Bibliographic Details
Main Author: University of Nebraska Lincoln (18796543)
Format: Dataset biblioteca
Published: 2019
Subjects:Genetics, raw sequence reads,
Online Access:https://figshare.com/articles/dataset/The_beef_cattle_rumen_microbiome_composition_is_influenced_by_host_genetics/25085600
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id dat-usda-us-article25085600
record_format figshare
spelling dat-usda-us-article250856002019-07-03T00:00:00Z The beef cattle rumen microbiome composition is influenced by host genetics University of Nebraska Lincoln (18796543) Genetics raw sequence reads DNA was extracted from the rumen samples using the PowerMag Soil DNA isolation kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol with a few modifications as described by publication. The modified protocol also included adding RNase A to the lysis solution to ensure removal of RNA during DNA extraction. The isolated DNA was stored at -20°C until used for bacterial community analysis. The V4 region of the 16S rRNA gene was amplified and sequenced on the MiSeq platform. 2019-07-03T00:00:00Z Dataset Dataset 10113/AF25085600 https://figshare.com/articles/dataset/The_beef_cattle_rumen_microbiome_composition_is_influenced_by_host_genetics/25085600 Copyright Undetermined
institution USDA US
collection Figshare
country Estados Unidos
countrycode US
component Datos de investigación
access En linea
databasecode dat-usda-us
tag biblioteca
region America del Norte
libraryname National Agricultural Library of USDA
topic Genetics
raw sequence reads
spellingShingle Genetics
raw sequence reads
University of Nebraska Lincoln (18796543)
The beef cattle rumen microbiome composition is influenced by host genetics
description DNA was extracted from the rumen samples using the PowerMag Soil DNA isolation kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol with a few modifications as described by publication. The modified protocol also included adding RNase A to the lysis solution to ensure removal of RNA during DNA extraction. The isolated DNA was stored at -20°C until used for bacterial community analysis. The V4 region of the 16S rRNA gene was amplified and sequenced on the MiSeq platform.
format Dataset
author University of Nebraska Lincoln (18796543)
author_facet University of Nebraska Lincoln (18796543)
author_sort University of Nebraska Lincoln (18796543)
title The beef cattle rumen microbiome composition is influenced by host genetics
title_short The beef cattle rumen microbiome composition is influenced by host genetics
title_full The beef cattle rumen microbiome composition is influenced by host genetics
title_fullStr The beef cattle rumen microbiome composition is influenced by host genetics
title_full_unstemmed The beef cattle rumen microbiome composition is influenced by host genetics
title_sort beef cattle rumen microbiome composition is influenced by host genetics
publishDate 2019
url https://figshare.com/articles/dataset/The_beef_cattle_rumen_microbiome_composition_is_influenced_by_host_genetics/25085600
work_keys_str_mv AT universityofnebraskalincoln18796543 thebeefcattlerumenmicrobiomecompositionisinfluencedbyhostgenetics
AT universityofnebraskalincoln18796543 beefcattlerumenmicrobiomecompositionisinfluencedbyhostgenetics
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