Sequencing of genome-edited wheat lines derived from cultivar Fielder
Wheat immunotoxicity is associated with abnormal reaction to gluten-derived peptides. Attempts to reduce immunotoxicity using breeding and biotechnology often affect dough quality. Here, the multiplexed CRISPR-Cas9 editing of cultivar Fielder was used to modify gluten-encoding genes, specifically focusing on omega- and gamma-gliadin gene copies, which were identified to be abundant in immunoreactive peptides based on the analysis of wheat genomes assembled using the long-read sequencing technologies. The whole genome sequencing of an edited line showed mutation or deletion of nearly all omega-gliadin and half of the gamma-gliadin gene copies and confirmed the lack of editing in the alpha/beta-gliadin genes. The estimated 75% and 64% reduction in omega- and gamma-gliadin content, respectively, had no negative impact on the end-use quality characteristics of grain protein and dough. A 47-fold immunoreactivity reduction compared to non-edited line was demonstrated using antibodies against immunotoxic peptides. Our results indicate that the targeted CRISPR-based modification of the omega- and gamma-gliadin gene copies determined to be abundant in immunoreactive peptides by analyzing high-quality genome assemblies is an effective mean for reducing immunotoxicity of wheat cultivars while minimizing the impact of editing on protein quality.
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| Format: | Dataset biblioteca |
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2023
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| Subjects: | Genetics, Triticum aestivum, eEukaryotes, raw sequence reads, genome sequencing, |
| Online Access: | https://figshare.com/articles/dataset/Sequencing_of_genome-edited_wheat_lines_derived_from_cultivar_Fielder/25078760 |
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dat-usda-us-article250787602023-11-12T00:00:00Z Sequencing of genome-edited wheat lines derived from cultivar Fielder Kansas State University (17480703) Genetics Triticum aestivum eEukaryotes raw sequence reads genome sequencing Wheat immunotoxicity is associated with abnormal reaction to gluten-derived peptides. Attempts to reduce immunotoxicity using breeding and biotechnology often affect dough quality. Here, the multiplexed CRISPR-Cas9 editing of cultivar Fielder was used to modify gluten-encoding genes, specifically focusing on omega- and gamma-gliadin gene copies, which were identified to be abundant in immunoreactive peptides based on the analysis of wheat genomes assembled using the long-read sequencing technologies. The whole genome sequencing of an edited line showed mutation or deletion of nearly all omega-gliadin and half of the gamma-gliadin gene copies and confirmed the lack of editing in the alpha/beta-gliadin genes. The estimated 75% and 64% reduction in omega- and gamma-gliadin content, respectively, had no negative impact on the end-use quality characteristics of grain protein and dough. A 47-fold immunoreactivity reduction compared to non-edited line was demonstrated using antibodies against immunotoxic peptides. Our results indicate that the targeted CRISPR-based modification of the omega- and gamma-gliadin gene copies determined to be abundant in immunoreactive peptides by analyzing high-quality genome assemblies is an effective mean for reducing immunotoxicity of wheat cultivars while minimizing the impact of editing on protein quality. 2023-11-12T00:00:00Z Dataset Dataset 10113/AF25078760 https://figshare.com/articles/dataset/Sequencing_of_genome-edited_wheat_lines_derived_from_cultivar_Fielder/25078760 Copyright Undetermined |
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Genetics Triticum aestivum eEukaryotes raw sequence reads genome sequencing |
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Genetics Triticum aestivum eEukaryotes raw sequence reads genome sequencing Kansas State University (17480703) Sequencing of genome-edited wheat lines derived from cultivar Fielder |
| description |
Wheat immunotoxicity is associated with abnormal reaction to gluten-derived peptides. Attempts to reduce immunotoxicity using breeding and biotechnology often affect dough quality. Here, the multiplexed CRISPR-Cas9 editing of cultivar Fielder was used to modify gluten-encoding genes, specifically focusing on omega- and gamma-gliadin gene copies, which were identified to be abundant in immunoreactive peptides based on the analysis of wheat genomes assembled using the long-read sequencing technologies. The whole genome sequencing of an edited line showed mutation or deletion of nearly all omega-gliadin and half of the gamma-gliadin gene copies and confirmed the lack of editing in the alpha/beta-gliadin genes. The estimated 75% and 64% reduction in omega- and gamma-gliadin content, respectively, had no negative impact on the end-use quality characteristics of grain protein and dough. A 47-fold immunoreactivity reduction compared to non-edited line was demonstrated using antibodies against immunotoxic peptides. Our results indicate that the targeted CRISPR-based modification of the omega- and gamma-gliadin gene copies determined to be abundant in immunoreactive peptides by analyzing high-quality genome assemblies is an effective mean for reducing immunotoxicity of wheat cultivars while minimizing the impact of editing on protein quality. |
| format |
Dataset |
| author |
Kansas State University (17480703) |
| author_facet |
Kansas State University (17480703) |
| author_sort |
Kansas State University (17480703) |
| title |
Sequencing of genome-edited wheat lines derived from cultivar Fielder |
| title_short |
Sequencing of genome-edited wheat lines derived from cultivar Fielder |
| title_full |
Sequencing of genome-edited wheat lines derived from cultivar Fielder |
| title_fullStr |
Sequencing of genome-edited wheat lines derived from cultivar Fielder |
| title_full_unstemmed |
Sequencing of genome-edited wheat lines derived from cultivar Fielder |
| title_sort |
sequencing of genome-edited wheat lines derived from cultivar fielder |
| publishDate |
2023 |
| url |
https://figshare.com/articles/dataset/Sequencing_of_genome-edited_wheat_lines_derived_from_cultivar_Fielder/25078760 |
| work_keys_str_mv |
AT kansasstateuniversity17480703 sequencingofgenomeeditedwheatlinesderivedfromcultivarfielder |
| _version_ |
1808945533337731072 |