Coffee protoplasts: isolation, culture and plantlet regeneration
Protoplasts have been isolated from cell suspensions of four coffee species: Coffea arabica cv. Catimor, C. canephora, C. racemosa and C. salvatrix. Isolation was performed in a mineral solution (0.33 M KCL, 0.04 M MgSO4, 6 mM CaCl2) with 1 per cent cellulase, 1 per cent Driselase, and 0,2 per cent pectinase. Cell wall regeneration and some cell divisions were observed in protoplast cultures. Regenerated cells survived up to the days. Somatic embryos which had developed in a cell suspension of C. canephora were used for protoplast isolation, too. The isolation medium contained 2 per cent cellulase, 1 per cent Driselase, 1 per cent Pectinol and 0.51 M glucose. Protoplasts were cultured in media supplemented with kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D) and napthaleneacetic acid (NAA), 0.5 mg/l of each. After a sequence of subcultures with decreasing glucose concentrations and after omission of growth regulators, embryos were formed. Some of these embryos could be regenerated to plantlets
| Main Authors: | , , , , |
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| Format: | biblioteca |
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París (Francia)
1988
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| Subjects: | COFFEA ARABICA, COFFEA CANEPHORA, COFFEA RACEMOSA, COFFEA SALVATRIX, PROTOPLASTOS, EMBRIOGENESIS SOMATICA, EXPLANTES, |
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| Summary: | Protoplasts have been isolated from cell suspensions of four coffee species: Coffea arabica cv. Catimor, C. canephora, C. racemosa and C. salvatrix. Isolation was performed in a mineral solution (0.33 M KCL, 0.04 M MgSO4, 6 mM CaCl2) with 1 per cent cellulase, 1 per cent Driselase, and 0,2 per cent pectinase. Cell wall regeneration and some cell divisions were observed in protoplast cultures. Regenerated cells survived up to the days. Somatic embryos which had developed in a cell suspension of C. canephora were used for protoplast isolation, too. The isolation medium contained 2 per cent cellulase, 1 per cent Driselase, 1 per cent Pectinol and 0.51 M glucose. Protoplasts were cultured in media supplemented with kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D) and napthaleneacetic acid (NAA), 0.5 mg/l of each. After a sequence of subcultures with decreasing glucose concentrations and after omission of growth regulators, embryos were formed. Some of these embryos could be regenerated to plantlets |
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