Structure fine du grain du café vert
A structural study has been carried out using optical and electron microscopy on green coffee beans, both fresh and dried, of the species C. canephora, var. robusta, originating from the Ivory Coast. Histochemical techniques, alone or combined with fluorescent and polarising microscopy, have been applied to frozen sections. These methods have enabled a detailed examination to be carried out of the cell walls, the detailed examination to be carried out of the cell walls, the cytoplasm and the epidermis of the parenchymatous cells of the cotyledons. It was also possible to localise various constituents of the above structures, e.g. proteins, polysaccharides, saturated, and non saturated lipids, free fatty acids, glycerides, chlorogenic acids, serotonin, lignin, pectic substances, various mineral salts, and caffeine (partially). This preliminary work was of assistance for a morte extensive examination of transmission electron microscopy. A new embedding material of transmission electronmicroscopy. A new embedding material (Spurr's mixture) greatly aided the production of ultra thin sections. The cell wall was shown in fine detail by the application of stains in very acid solutions of
| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
Paris (Francia)
1979
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| Subjects: | COFFEA CANEPHORA, ANATOMIA DE LA PLANTA, FRUTO, ROBUSTA, GRANOS, COMPOSICION DEL GRANO, ANATOMIA DEL GRANO, |
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| Summary: | A structural study has been carried out using optical and electron microscopy on green coffee beans, both fresh and dried, of the species C. canephora, var. robusta, originating from the Ivory Coast. Histochemical techniques, alone or combined with fluorescent and polarising microscopy, have been applied to frozen sections. These methods have enabled a detailed examination to be carried out of the cell walls, the detailed examination to be carried out of the cell walls, the cytoplasm and the epidermis of the parenchymatous cells of the cotyledons. It was also possible to localise various constituents of the above structures, e.g. proteins, polysaccharides, saturated, and non saturated lipids, free fatty acids, glycerides, chlorogenic acids, serotonin, lignin, pectic substances, various mineral salts, and caffeine (partially). This preliminary work was of assistance for a morte extensive examination of transmission electron microscopy. A new embedding material of transmission electronmicroscopy. A new embedding material (Spurr's mixture) greatly aided the production of ultra thin sections. The cell wall was shown in fine detail by the application of stains in very acid solutions of |
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