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Moniliophthora roreri is the causal agent for Monilia pod rot, a fungal disease that causes important loses in cocoa production in Central American countries. The present work studied restriction fragment length polymorphism (RFLP) of tolerant and susceptibles cocoa plants to analyze the advantages of this methodology for the characterization of both groups. The resistant clones studied were: CC-137, EET-67, EET-75, EET-183 and UF-273; and the susceptible ones were: POUND-7, CC-132, UF-29, UF-221, CATONGO and UF-613. The use of only one probe (21 KD) in combination with one restriction enzime (Hae III) led to the separation of six defined groups between the different cocoa clones used in this study. This result demonstrate the potentiality of the RFLP analysis for the genetic characterization of tolerant clones.

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Bibliographic Details
Main Authors: 66690 Febres Rodríguez, V.J., 85214 Lastra, R., 2664 American Phytopathological Society, St. Paul, Minn. (EUA). Caribbean Div., 2858 Asociación Costarricense de Fitopatología, San José (Costa Rica), 33182 31. Reunión Anual Sociedad Americana de Fitopatología. División del Caribe San José (Costa Rica) 20-25 May 1991
Format: biblioteca
Published: San José (Costa Rica) 1991
Subjects:THEOBROMA CACAO, CLONES, MONILIOPHTHORA RORERI, ENFERMEDADES FUNGOSAS, RESISTENCIA A LA ENFERMEDAD, POLIMORFISMO,
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Summary:Moniliophthora roreri is the causal agent for Monilia pod rot, a fungal disease that causes important loses in cocoa production in Central American countries. The present work studied restriction fragment length polymorphism (RFLP) of tolerant and susceptibles cocoa plants to analyze the advantages of this methodology for the characterization of both groups. The resistant clones studied were: CC-137, EET-67, EET-75, EET-183 and UF-273; and the susceptible ones were: POUND-7, CC-132, UF-29, UF-221, CATONGO and UF-613. The use of only one probe (21 KD) in combination with one restriction enzime (Hae III) led to the separation of six defined groups between the different cocoa clones used in this study. This result demonstrate the potentiality of the RFLP analysis for the genetic characterization of tolerant clones.