The use of isozyme analysis in the characterization of cocoa germplasm
Starch gel electrophoresis was performed on bark tissue of Theobroma cacao to generate isoperoxidase banding patterns. The zymograms obtained were used to broadly classify the extensive germplasm collection at the Cocoa Research Unit. Work done on other enzyme systems, a.g. malate dehydrogenase, alcohol dehydrogenase, laccase and phosphoglum comutase is expected to further subdivide the groups already established by peroxidase. The results so far indicate that there are differences in the banding patterns of the sample clones. There is also variation within populations originating from the same geographical site. These patterns will be used to uniquely characterise small groups of related germplasm and assist in a rational exploitation of genetic resources.
| Main Authors: | , , , , , |
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| Format: | biblioteca |
| Published: |
St. Augustine (Trinidad y Tobago)
1989
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| Subjects: | THEOBROMA CACAO, GERMOPLASMA, ISOENZIMAS, ANALISIS DE TEJIDOS, COLECCION DE PLANTAS, TRINIDAD Y TOBAGO, |
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| Summary: | Starch gel electrophoresis was performed on bark tissue of Theobroma cacao to generate isoperoxidase banding patterns. The zymograms obtained were used to broadly classify the extensive germplasm collection at the Cocoa Research Unit. Work done on other enzyme systems, a.g. malate dehydrogenase, alcohol dehydrogenase, laccase and phosphoglum comutase is expected to further subdivide the groups already established by peroxidase. The results so far indicate that there are differences in the banding patterns of the sample clones. There is also variation within populations originating from the same geographical site. These patterns will be used to uniquely characterise small groups of related germplasm and assist in a rational exploitation of genetic resources. |
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