In vitro germination and growth of cacao pollen
This paper deals with pollen germination, pollen tube growth and pollen tube mitosis in cacao (Theobroma cacao L.). Good pollen germination and growth were obtained in a medium containing 15 per cent sucrose, 100 ppm boric acid, and 100 ppm calcium nitrate. Boron or calcium was necessary for proper pollen germination and tube growth, and both, if present, enhanced tube growth considerable. No pollen germination was observed at low temperatures (10 centigrade grade), and maximum germination and tube growth were obtained at 35 centigrade grade. Further increase in temperature to 40 centigrade grade affected adversely both germination and tube growth. Pollen tube mitosis began within 2 1/2 hr of the initiation of pollen germination, and completed in 2 1/2 hr. Pollen tube mitosis was normal in the cultivars studied
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| Format: | biblioteca |
| Published: |
1977
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| Subjects: | THEOBROMA CACAO, POLEN, GERMINACION, CULTIVO IN VITRO, BORO, SUCROSA, ACIDO BORICO, TEMPERATURA, |
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| Summary: | This paper deals with pollen germination, pollen tube growth and pollen tube mitosis in cacao (Theobroma cacao L.). Good pollen germination and growth were obtained in a medium containing 15 per cent sucrose, 100 ppm boric acid, and 100 ppm calcium nitrate. Boron or calcium was necessary for proper pollen germination and tube growth, and both, if present, enhanced tube growth considerable. No pollen germination was observed at low temperatures (10 centigrade grade), and maximum germination and tube growth were obtained at 35 centigrade grade. Further increase in temperature to 40 centigrade grade affected adversely both germination and tube growth. Pollen tube mitosis began within 2 1/2 hr of the initiation of pollen germination, and completed in 2 1/2 hr. Pollen tube mitosis was normal in the cultivars studied |
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