Anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction

Anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction

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Immunity against Brucella, a facultative intracellular bacteria, can be induced in mice by live or by killed-whole-cell or fraction vaccine. This immunity can be adoptively transferred from live vaccinated donor to recipient mice, or passively with immune serum raised against bacterial fractions. A protein-bound peptidoglycan fraction (PG) was used to immunize DBA/2 donor mice intravenously or subcutaneously in the hind footpads. Splenic or popliteal lymph nodes cells were transferred to recipients that were intravenously challenged with a B. abortus virulent strain. Brucella spleen counts, and in some cases liver counts, were done 15 days later or at successive times. Spleen cells depleted from macrophages and lymph nodes cells conferred immunity to recipients. This immunity increased after transfer for about 21 days. Lymph nodes cells were more efficient to transfer immunity than spleen cells. This immunity was abated but not abrogated by treatment of cells anti-Thy serum and was transferred by both nylon wool non adherent (T enriched) or adherent (B enriched) cells. Comparative transfer experiments with DBA/2 versus CBA mice, live vaccine versus PG fraction vaccine, virulent versus a virulent (vaccinal) challenge evidenced that the three factors were involved in induction and/or expression of immunity. DBA/2 mice responded better to fraction vaccine and virulent challenge whereas CBA responded better to live vaccine and to a virulent challenge strain. In contrast DBA/2 x CBA F1 responded equally well against the virulent challenge to transfer of cells from live vaccine or PG fraction vaccinated donor mice. Two cell-mediated immune mechanisms may thus be involved in transferred immunity the expressions of which may be genetically determined

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Main Authors: 105945 Plommet, M., 105944 Plommet, A.M.
Format: biblioteca
Published: 1987
Subjects:BACTERIOSIS, BRUCELOSIS, BRUCELLA ABORTUS, INMUNIZACION,
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spelling KOHA-OAI-BVE:424382020-02-03T21:05:43ZAnti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction 105945 Plommet, M. 105944 Plommet, A.M. 1987Immunity against Brucella, a facultative intracellular bacteria, can be induced in mice by live or by killed-whole-cell or fraction vaccine. This immunity can be adoptively transferred from live vaccinated donor to recipient mice, or passively with immune serum raised against bacterial fractions. A protein-bound peptidoglycan fraction (PG) was used to immunize DBA/2 donor mice intravenously or subcutaneously in the hind footpads. Splenic or popliteal lymph nodes cells were transferred to recipients that were intravenously challenged with a B. abortus virulent strain. Brucella spleen counts, and in some cases liver counts, were done 15 days later or at successive times. Spleen cells depleted from macrophages and lymph nodes cells conferred immunity to recipients. This immunity increased after transfer for about 21 days. Lymph nodes cells were more efficient to transfer immunity than spleen cells. This immunity was abated but not abrogated by treatment of cells anti-Thy serum and was transferred by both nylon wool non adherent (T enriched) or adherent (B enriched) cells. Comparative transfer experiments with DBA/2 versus CBA mice, live vaccine versus PG fraction vaccine, virulent versus a virulent (vaccinal) challenge evidenced that the three factors were involved in induction and/or expression of immunity. DBA/2 mice responded better to fraction vaccine and virulent challenge whereas CBA responded better to live vaccine and to a virulent challenge strain. In contrast DBA/2 x CBA F1 responded equally well against the virulent challenge to transfer of cells from live vaccine or PG fraction vaccinated donor mice. Two cell-mediated immune mechanisms may thus be involved in transferred immunity the expressions of which may be genetically determinedImmunity against Brucella, a facultative intracellular bacteria, can be induced in mice by live or by killed-whole-cell or fraction vaccine. This immunity can be adoptively transferred from live vaccinated donor to recipient mice, or passively with immune serum raised against bacterial fractions. A protein-bound peptidoglycan fraction (PG) was used to immunize DBA/2 donor mice intravenously or subcutaneously in the hind footpads. Splenic or popliteal lymph nodes cells were transferred to recipients that were intravenously challenged with a B. abortus virulent strain. Brucella spleen counts, and in some cases liver counts, were done 15 days later or at successive times. Spleen cells depleted from macrophages and lymph nodes cells conferred immunity to recipients. This immunity increased after transfer for about 21 days. Lymph nodes cells were more efficient to transfer immunity than spleen cells. This immunity was abated but not abrogated by treatment of cells anti-Thy serum and was transferred by both nylon wool non adherent (T enriched) or adherent (B enriched) cells. Comparative transfer experiments with DBA/2 versus CBA mice, live vaccine versus PG fraction vaccine, virulent versus a virulent (vaccinal) challenge evidenced that the three factors were involved in induction and/or expression of immunity. DBA/2 mice responded better to fraction vaccine and virulent challenge whereas CBA responded better to live vaccine and to a virulent challenge strain. In contrast DBA/2 x CBA F1 responded equally well against the virulent challenge to transfer of cells from live vaccine or PG fraction vaccinated donor mice. Two cell-mediated immune mechanisms may thus be involved in transferred immunity the expressions of which may be genetically determinedBACTERIOSISBRUCELOSISBRUCELLA ABORTUSINMUNIZACIONAnnales de Recherches Veterinaires (Francia)
institution IICA
collection Koha
country Costa Rica
countrycode CR
component Bibliográfico
access En linea
databasecode cat-sibiica
tag biblioteca
region America Central
libraryname Sistema de Bibliotecas IICA/CATIE
topic BACTERIOSIS
BRUCELOSIS
BRUCELLA ABORTUS
INMUNIZACION
BACTERIOSIS
BRUCELOSIS
BRUCELLA ABORTUS
INMUNIZACION
spellingShingle BACTERIOSIS
BRUCELOSIS
BRUCELLA ABORTUS
INMUNIZACION
BACTERIOSIS
BRUCELOSIS
BRUCELLA ABORTUS
INMUNIZACION
105945 Plommet, M.
105944 Plommet, A.M.
Anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction
description Immunity against Brucella, a facultative intracellular bacteria, can be induced in mice by live or by killed-whole-cell or fraction vaccine. This immunity can be adoptively transferred from live vaccinated donor to recipient mice, or passively with immune serum raised against bacterial fractions. A protein-bound peptidoglycan fraction (PG) was used to immunize DBA/2 donor mice intravenously or subcutaneously in the hind footpads. Splenic or popliteal lymph nodes cells were transferred to recipients that were intravenously challenged with a B. abortus virulent strain. Brucella spleen counts, and in some cases liver counts, were done 15 days later or at successive times. Spleen cells depleted from macrophages and lymph nodes cells conferred immunity to recipients. This immunity increased after transfer for about 21 days. Lymph nodes cells were more efficient to transfer immunity than spleen cells. This immunity was abated but not abrogated by treatment of cells anti-Thy serum and was transferred by both nylon wool non adherent (T enriched) or adherent (B enriched) cells. Comparative transfer experiments with DBA/2 versus CBA mice, live vaccine versus PG fraction vaccine, virulent versus a virulent (vaccinal) challenge evidenced that the three factors were involved in induction and/or expression of immunity. DBA/2 mice responded better to fraction vaccine and virulent challenge whereas CBA responded better to live vaccine and to a virulent challenge strain. In contrast DBA/2 x CBA F1 responded equally well against the virulent challenge to transfer of cells from live vaccine or PG fraction vaccinated donor mice. Two cell-mediated immune mechanisms may thus be involved in transferred immunity the expressions of which may be genetically determined
format
topic_facet BACTERIOSIS
BRUCELOSIS
BRUCELLA ABORTUS
INMUNIZACION
author 105945 Plommet, M.
105944 Plommet, A.M.
author_facet 105945 Plommet, M.
105944 Plommet, A.M.
author_sort 105945 Plommet, M.
title Anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction
title_short Anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction
title_full Anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction
title_fullStr Anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction
title_full_unstemmed Anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction
title_sort anti-brucella cell-mediated immunity in mice vaccinated with a cell-wall fraction
publishDate 1987
work_keys_str_mv AT 105945plommetm antibrucellacellmediatedimmunityinmicevaccinatedwithacellwallfraction
AT 105944plommetam antibrucellacellmediatedimmunityinmicevaccinatedwithacellwallfraction
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