Method validation and determination of heavy metals in cocoa beans and cocoa products by microwave assisted digestion technique with inductively coupled plasma mass spectrometry
In this article, an easy and quick method based on microwave assisted acid digestion technique prior to quantification using inductively coupled plasma mass spectrometry for the analysis of heavy metals in cocoa beans, cocoa powder and chocolate was established and validated for arsenic (As), cadmium (Cd), lead (Pb), and antimony (Sb). Limit of quantification for all elements were product dependent and varies from 7.84 to 194.52 μg/kg. The recoveries of the heavy metals at 250 and 1000 μg/kg spiking levels were ranged between 96.27–108.75%, 90.43–101.97% and 89.72–106.26% for cocoa beans, cocoa powder, and chocolate, respectively. Relative standard deviation values obtained were all below 20% and the expanded uncertainty measurements for the elements were less than 25%. The analysis of real samples found that the concentration level is far from the national alarming level except for cadmium in cocoa beans.
| Main Authors: | , , |
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| Format: | Texto biblioteca |
| Language: | a d |
| Published: |
Amsterdam, Netherlands Elsevier
2020
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| Subjects: | theobroma cacao, measurement uncertainty, heavy metals, microwave acid-digestion, ICP-MS, |
| Online Access: | https://drive.google.com/drive/folders/1_sUzVGrTvSzgzL7TsMcorwAZR2mp6MqM?usp=sharing |
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| Summary: | In this article, an easy and quick method based on microwave assisted acid digestion technique prior to quantification
using inductively coupled plasma mass spectrometry for the analysis of heavy metals in cocoa beans,
cocoa powder and chocolate was established and validated for arsenic (As), cadmium (Cd), lead (Pb), and
antimony (Sb). Limit of quantification for all elements were product dependent and varies from 7.84 to
194.52 μg/kg. The recoveries of the heavy metals at 250 and 1000 μg/kg spiking levels were ranged between
96.27–108.75%, 90.43–101.97% and 89.72–106.26% for cocoa beans, cocoa powder, and chocolate, respectively.
Relative standard deviation values obtained were all below 20% and the expanded uncertainty measurements
for the elements were less than 25%. The analysis of real samples found that the concentration level is
far from the national alarming level except for cadmium in cocoa beans. |
|---|