Activation of a calcium - dependent protein kinase involved in the Azospirillum growth promotion in rice
Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident. An increase in the Ca2 plus sign dependent histone kinase activity was also detected as a result of inoculation. The biochemical characterization and Western blot analysis of the kinase strongly supports the hypothesis that it belongs to a member of the rice CDPK family. The fact that the amount of the protein did not change upon inoculation seems to indicate that a posttranslational activation is responsible for the change in the enzymatic activity. An in-gel kinase experiment identified a 46 kDa CDPK like protein kinase as a putative component of the signal transduction pathway triggered by Azospirillum inoculation. To our knowledge, this is the first report on the possible involvement of a Ca2 plus sign dependent protein kinase in promotion of rice plants growth by A. brasilense.
| Main Authors: | , , |
|---|---|
| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | AZOSPIRILLUM, RICE, CDPK, LATERAL ROOTS, GROWTH PROMOTION, |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45775 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident.
An increase in the Ca2 plus sign dependent histone kinase activity was also detected as a result of inoculation.
The biochemical characterization and Western blot analysis of the kinase strongly supports the hypothesis that it belongs to a member of the rice CDPK family.
The fact that the amount of the protein did not change upon inoculation seems to indicate that a posttranslational activation is responsible for the change in the enzymatic activity. An in-gel kinase experiment identified a 46 kDa CDPK like protein kinase as a putative component of the signal transduction pathway triggered by Azospirillum inoculation.
To our knowledge, this is the first report on the possible involvement of a Ca2 plus sign dependent protein kinase in promotion of rice plants growth by A. brasilense. |
|---|