Optimization of tissue culture conditions and in vitro and in planta transformation of the local variety of rice Don Justo FCAyF
Rice (Oryza sativa L.) is one of the most economically important cereals because it provides food for more than half of the world's population. Improving this crop through genetic engineering requires fine-tuning processes such as tissue culture and transformation. Here, we optimized tissue in vitro culture and transformation methods of the local premium cultivar Don Justo FCAyF. Regarding tissue culture, the optimal 2,4D concentration was 3 mg.l-1showing 42% of callogenesis. Shoot regeneration showed 77% efficiency using a combination of 4 mg.l-1 BAP and 0.8 mg.l-1 NAA. About Agrobacterium tumefaciens transformation we obtained variable results depending on the plasmid as well as the explant used. In the case of in vitro transformation of embryogenic calli, values between 14-29% were obtained depending on the plasmid. While, in planta methods of embryo transformation presented an efficiency between 3-28%. In conclusion, in this work the methods for tissue cultivation and Agrobacterium transformation of a local variety of rice were fine-tuned. These results will facilitate its genetic improvement through different techniques such as conventional transgenesis or gene editing.
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata
2022
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| Online Access: | https://revistas.unlp.edu.ar/revagro/article/view/14143 |
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