Lymph nodes and ground beef as public health importance reservoirs of Salmonella spp.
This study aimed to determine the frequency of contamination, serovar diversity, and multilocus sequence typing (MLST) of Salmonella enterica (SE) in lymph nodes and ground beef. A total of 1,545 samples from 400 beef carcasses were analyzed. Samples included peripheral (PLN) and deep lymph nodes (DLN), lean and fatty ground beef obtained in warm (April-July) and cold (September-December) seasons during 2017 and 2018. The pure isolates were subjected to complete genome sequencing. With these data, the in silico prediction of serovars and the MLST profile was performed. In total, 78 SE isolates were obtained (5 % of the total analyzed samples). The frequency of contamination was associated with the type of sample ( 2=23.7, P<0.0001) and the time of year ( 2=20.3, P<0.0001), being higher in PLN (9.7%) and during the warm season (7.0%). The predominant serovars were Anatum and Reading (each one with n= 23), Typhimurium (n= 11), and London (n= 9). The MLST profile of strains of the Typhimurium (ST 19 and 34) and Kentucky (ST 198) serovars has been previously reported in isolates involved in clinical cases. It was concluded that lymph nodes and ground beef are reservoirs of SE of public health importance, especially during the warm months of the year. Therefore, it is necessary to establish measures to prevent dissemination throughout the production chain of strains associated with apparently healthy animals.
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Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2020
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Online Access: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5516 |
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Palós Gutiérrez, Tania Rubio Lozano, María Salud Delgado Suárez, Enrique Jesús Rosi Guzmán, Naisy Soberanis Ramos, Orbelin Hernández Pérez, Cindy Fabiola Méndez Medina, Rubén Danilo |
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Palós Gutiérrez, Tania Rubio Lozano, María Salud Delgado Suárez, Enrique Jesús Rosi Guzmán, Naisy Soberanis Ramos, Orbelin Hernández Pérez, Cindy Fabiola Méndez Medina, Rubén Danilo Lymph nodes and ground beef as public health importance reservoirs of Salmonella spp. |
author_facet |
Palós Gutiérrez, Tania Rubio Lozano, María Salud Delgado Suárez, Enrique Jesús Rosi Guzmán, Naisy Soberanis Ramos, Orbelin Hernández Pérez, Cindy Fabiola Méndez Medina, Rubén Danilo |
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Palós Gutiérrez, Tania |
title |
Lymph nodes and ground beef as public health importance reservoirs of Salmonella spp. |
title_short |
Lymph nodes and ground beef as public health importance reservoirs of Salmonella spp. |
title_full |
Lymph nodes and ground beef as public health importance reservoirs of Salmonella spp. |
title_fullStr |
Lymph nodes and ground beef as public health importance reservoirs of Salmonella spp. |
title_full_unstemmed |
Lymph nodes and ground beef as public health importance reservoirs of Salmonella spp. |
title_sort |
lymph nodes and ground beef as public health importance reservoirs of salmonella spp. |
description |
This study aimed to determine the frequency of contamination, serovar diversity, and multilocus sequence typing (MLST) of Salmonella enterica (SE) in lymph nodes and ground beef. A total of 1,545 samples from 400 beef carcasses were analyzed. Samples included peripheral (PLN) and deep lymph nodes (DLN), lean and fatty ground beef obtained in warm (April-July) and cold (September-December) seasons during 2017 and 2018. The pure isolates were subjected to complete genome sequencing. With these data, the in silico prediction of serovars and the MLST profile was performed. In total, 78 SE isolates were obtained (5 % of the total analyzed samples). The frequency of contamination was associated with the type of sample ( 2=23.7, P<0.0001) and the time of year ( 2=20.3, P<0.0001), being higher in PLN (9.7%) and during the warm season (7.0%). The predominant serovars were Anatum and Reading (each one with n= 23), Typhimurium (n= 11), and London (n= 9). The MLST profile of strains of the Typhimurium (ST 19 and 34) and Kentucky (ST 198) serovars has been previously reported in isolates involved in clinical cases. It was concluded that lymph nodes and ground beef are reservoirs of SE of public health importance, especially during the warm months of the year. Therefore, it is necessary to establish measures to prevent dissemination throughout the production chain of strains associated with apparently healthy animals. |
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Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias |
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2020 |
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https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5516 |
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rev-remexcp-article55162022-08-16T18:53:37Z Lymph nodes and ground beef as public health importance reservoirs of Salmonella spp. Linfonodos y carne molida de res como reservorios de Salmonella spp. de importancia en salud pública Palós Gutiérrez, Tania Rubio Lozano, María Salud Delgado Suárez, Enrique Jesús Rosi Guzmán, Naisy Soberanis Ramos, Orbelin Hernández Pérez, Cindy Fabiola Méndez Medina, Rubén Danilo Salmonella; cattle; lymph nodes; ground beef; Serovars; MLST Salmonella; bovinos; linfonodos; carne molida; serotipos, MLST This study aimed to determine the frequency of contamination, serovar diversity, and multilocus sequence typing (MLST) of Salmonella enterica (SE) in lymph nodes and ground beef. A total of 1,545 samples from 400 beef carcasses were analyzed. Samples included peripheral (PLN) and deep lymph nodes (DLN), lean and fatty ground beef obtained in warm (April-July) and cold (September-December) seasons during 2017 and 2018. The pure isolates were subjected to complete genome sequencing. With these data, the in silico prediction of serovars and the MLST profile was performed. In total, 78 SE isolates were obtained (5 % of the total analyzed samples). The frequency of contamination was associated with the type of sample ( 2=23.7, P<0.0001) and the time of year ( 2=20.3, P<0.0001), being higher in PLN (9.7%) and during the warm season (7.0%). The predominant serovars were Anatum and Reading (each one with n= 23), Typhimurium (n= 11), and London (n= 9). The MLST profile of strains of the Typhimurium (ST 19 and 34) and Kentucky (ST 198) serovars has been previously reported in isolates involved in clinical cases. It was concluded that lymph nodes and ground beef are reservoirs of SE of public health importance, especially during the warm months of the year. Therefore, it is necessary to establish measures to prevent dissemination throughout the production chain of strains associated with apparently healthy animals. El objetivo fue estimar la frecuencia de contaminación, diversidad de serotipos y tipificación por multilocus (MLST) de Salmonella enterica (SE) en linfonodos y carne molida de bovino. Para ello, se analizaron 1545 muestras provenientes de 400 canales bovinas. El muestreo incluyó linfonodos superficiales (LNS) y profundos, carne molida magra y con grasa, obtenidas en estación cálida (abril-julio) y fría (septiembre-diciembre) durante 2017 y 2018. Los aislamientos puros se sometieron a secuenciación completa del genoma. Con estos datos, se realizó la predicción in silico de serotipos y del perfil de MLST. En total, se obtuvieron 78 aislamientos de SE (5% del total de muestras analizadas). La frecuencia de contaminación se asoció con el tipo de muestra (2=23.7, P<0.0001) y con la época del año (2=20.3, P<0.0001), siendo mayor en LNS (9.7%) y en estación cálida (7.0%). Los serotipos predominantes fueron Anatum y Reading (n=23 cada uno), Typhimurium (n=11) y London (n=9). El perfil de MLST de cepas de los serotipos Typhimurium (ST 19 y 34) y Kentucky (ST 198) se ha reportado previamente en aislamientos involucrados en casos clínicos. Se concluye que los linfonodos y la carne molida de res son reservorios de SE de importancia en salud pública, especialmente durante los meses cálidos del año. Por tanto, es necesario establecer medidas encaminadas a prevenir la diseminación, a lo largo de la cadena productiva, de las cepas asociadas con animales aparentemente sanos. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias Departamento de Secuenciación y Bioinformatica del Centro Nacional de Referencia de Plaguicidas y Contaminantes del Senasica y el Departamento de Medicina Preventiva y Salud Pública de la FMVZ de la UNAM 2020-09-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf text/html https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5516 10.22319/rmcp.v11i3.5516 Revista Mexicana de Ciencias Pecuarias; Vol. 11, Núm. 3 (2020): Julio-Septiembre; 795-810 Revista Mexicana de Ciencias Pecuarias; Vol. 11, Núm. 3 (2020): Julio-Septiembre; 795-810 2448-6698 2007-1124 spa eng https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5516/4426 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5516/4427 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5516/4979 http://creativecommons.org/licenses/by-nc-sa/4.0 |