Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)

Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)

To study the genetic diversity of the national collection of Trichanthera gigantea (Humb. & Bonpl.) Nees from the Foundation Center for Research in Sustainable Systems for Agricultural Production (CIPAV), five primers RAMs were used on 38 accessions from 11 departments of Colombia and two states of Venezuela. A pattern of 71 bands was obtained and used to estimate genetic diversity parameters. A specific protocol for DNA isolation was adapted and a DNA bank was established at the National University of Colombia, Palmira campus for further investigations. The results showed heterogeneity and high genetic diversity in these accessions. No relationship was found among molecular group analysis and the geographical origin zones.

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Main Authors: Posso Terranova, Andrés Mauricio, Cárdenas Henao, Heiber, Murgueitio, Enrique, Leterme, Pascal, Muñoz Flórez, Jaime Eduardo
Format: Digital revista
Language:spa
Published: Universidad Nacional de Colombia - Sede Palmira 2011
Online Access:https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27834
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spelling oai:www.revistas.unal.edu.co:article-278342014-06-21T23:41:16Z Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites) Diversidad genética de accesiones de nacedero Trichanthera gigantea (Humb. & Bonpl.) Nees, mediante RAM’s (Random Amplified Microsatellites) Posso Terranova, Andrés Mauricio Cárdenas Henao, Heiber Murgueitio, Enrique Leterme, Pascal Muñoz Flórez, Jaime Eduardo DNA DNA isolation genetic markers germplasm banks nacedero Trichanthera gigantea. ADN bancos de germoplasma extracción de ADN marcadores genéticos nacedero Trichanthera gigantea. To study the genetic diversity of the national collection of Trichanthera gigantea (Humb. & Bonpl.) Nees from the Foundation Center for Research in Sustainable Systems for Agricultural Production (CIPAV), five primers RAMs were used on 38 accessions from 11 departments of Colombia and two states of Venezuela. A pattern of 71 bands was obtained and used to estimate genetic diversity parameters. A specific protocol for DNA isolation was adapted and a DNA bank was established at the National University of Colombia, Palmira campus for further investigations. The results showed heterogeneity and high genetic diversity in these accessions. No relationship was found among molecular group analysis and the geographical origin zones. Con el objeto de estudiar la diversidad genética del árbol forrajero nacedero Trichanthera gigantea (Humb. Bonpl.) Nees, existente en la colección nacional de la Fundación Centro para la Investigación en Sistemas Sostenibles de Producción Agropecuaria (CIPAV) se utilizaron cinco cebadores RAMs en 38 accesiones procedentes de once departamentos de Colombia y dos estados de Venezuela. Se obtuvieron 71 bandas usadas para estimar los parámetros de diversidad genética. Se adaptó un protocolo de extracción de ADN para la especie y se estableció un banco de ADN de las accesiones en la Universidad Nacional de Colombia sede Palmira, para la realización de trabajos futuros. Los resultados indicaron que la colección nacional de nacedero presenta heterogeneidad en sus accesiones, alta diversidad genética y no se encontró relación entre los grupos genéticos formados mediante el análisis molecular y las zonas geográficas de origen. Universidad Nacional de Colombia - Sede Palmira 2011-04-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/msword application/pdf text/html https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27834 Acta Agronómica; Vol. 60 No. 2 (2011); 120-131 Acta Agronómica; Vol. 60 Núm. 2 (2011); 120-131 Acta Agronómica; v. 60 n. 2 (2011); 120-131 2323-0118 0120-2812 spa https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27834/28080 https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27834/28081 https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27834/28082 Derechos de autor 2011 Acta Agronómica https://creativecommons.org/licenses/by-nc-nd/4.0
institution UNAL
collection OJS
country Colombia
countrycode CO
component Revista
access En linea
databasecode rev-actaagronomica
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region America del Sur
libraryname Sistema Nacional de Bibliotecas de la UNAL
language spa
format Digital
author Posso Terranova, Andrés Mauricio
Cárdenas Henao, Heiber
Murgueitio, Enrique
Leterme, Pascal
Muñoz Flórez, Jaime Eduardo
spellingShingle Posso Terranova, Andrés Mauricio
Cárdenas Henao, Heiber
Murgueitio, Enrique
Leterme, Pascal
Muñoz Flórez, Jaime Eduardo
Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)
author_facet Posso Terranova, Andrés Mauricio
Cárdenas Henao, Heiber
Murgueitio, Enrique
Leterme, Pascal
Muñoz Flórez, Jaime Eduardo
author_sort Posso Terranova, Andrés Mauricio
title Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)
title_short Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)
title_full Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)
title_fullStr Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)
title_full_unstemmed Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)
title_sort genetic diversity of accessions of trichanthera gigantea (humb. & bonpl.) nees, using rams (random amplified microsatellites)
description To study the genetic diversity of the national collection of Trichanthera gigantea (Humb. & Bonpl.) Nees from the Foundation Center for Research in Sustainable Systems for Agricultural Production (CIPAV), five primers RAMs were used on 38 accessions from 11 departments of Colombia and two states of Venezuela. A pattern of 71 bands was obtained and used to estimate genetic diversity parameters. A specific protocol for DNA isolation was adapted and a DNA bank was established at the National University of Colombia, Palmira campus for further investigations. The results showed heterogeneity and high genetic diversity in these accessions. No relationship was found among molecular group analysis and the geographical origin zones.
publisher Universidad Nacional de Colombia - Sede Palmira
publishDate 2011
url https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27834
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