Utilización del patrón de restricción del DNA codificante para el RNA Ribosomal de la subunidad pequeña para la caracterización de Apicomplexa
Apicomplexa is a Protozoa phylum in which all members are obliged parasites of a wide range of vertebrate and invertebrate hosts. There is an ongoing controversy onsystematics, phylogenetic relationships and parasite - host coevolution patterns. The SSU ribosomal gen has been used as a molecular marker in order to solve these issues.From DNA of the species of the Sarcocystidae family (Sarcocystis cruzi, Sarcocystis sp. from Didelphis marsupialis, Sarcocystis sp. from Columbina talpacoti and Toxoplasmagondii), and from the species of the Plasmodiidae family (Plasmodium from Anolis ehloris, P. simium, and P. falciparum), the SSU ribosomal DNA fragemnt wasamplified by PCR, using the pair of primers P5-P3, 0009-2134 and 566R-567R. Hind III, Eco RI, Sau 3AI and Alw 261 restriction pattems were compared. It was shownthat riboprinting analysis was able to discriminate between these Apicomplexa families and at the same time detected differences between genus and intraespecific variants.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
1996
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| Online Access: | https://revistas.unal.edu.co/index.php/actabiol/article/view/20723 |
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