METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)

ABSTRACT The objective of this work was to evaluate Fusarium guttiforme inoculation methods and genetic resistance of pineapple accessions. Thus, three experiments were conducted: pathogen inoculation of different leaf types (B, D and F) of pineapple (1), pathogen inoculation of pineapple cuttings and detached D leaves (2), and identification of resistance to fusariosis in 19 pineapple accessions (3) sampled in the State of Mato Grosso, Brazil. The cultivars Pérola (susceptible to fusariosis) and BRS-Vitória (resistant to fusariosis) were used as controls. The fusariosis severity was evaluated at 10, 15, 20, 25 and 30 days after inoculation with F. guttiforme. The lesion diameters (severity level) were used in order to calculate the area under the disease progress curve (AUDPC). The inoculation of detached D leaves was the most efficient, fast and inexpensive method, and the one that most satisfactorily reproduced the disease symptoms. The period of 10 to 20 days after inoculation of the D detached leaves with the pathogen is the most suitable to evaluate the resistance of pineapple accessions to fusariosis. The lowest lesion area and AUDPC was found in the accession 1, in all evaluations. Thus, the accession 1 can be used in pineapple breeding programs for resistance to fusariosis.

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Main Authors: GARCIA,WANDREILLA MOREIRA, KRAUSE,WILLIAN, ARAÚJO,DEJÂNIA VIEIRA DE, KARSBURG,ISANE VERA, DALLACORT,RIVANILDO
Format: Digital revista
Language:English
Published: Universidade Federal Rural do Semi-Árido 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-21252017000200353
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spelling oai:scielo:S1983-212520170002003532017-04-12METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)GARCIA,WANDREILLA MOREIRAKRAUSE,WILLIANARAÚJO,DEJÂNIA VIEIRA DEKARSBURG,ISANE VERADALLACORT,RIVANILDO Ananas comosus var comosus Genetic resistance Fusariosis. ABSTRACT The objective of this work was to evaluate Fusarium guttiforme inoculation methods and genetic resistance of pineapple accessions. Thus, three experiments were conducted: pathogen inoculation of different leaf types (B, D and F) of pineapple (1), pathogen inoculation of pineapple cuttings and detached D leaves (2), and identification of resistance to fusariosis in 19 pineapple accessions (3) sampled in the State of Mato Grosso, Brazil. The cultivars Pérola (susceptible to fusariosis) and BRS-Vitória (resistant to fusariosis) were used as controls. The fusariosis severity was evaluated at 10, 15, 20, 25 and 30 days after inoculation with F. guttiforme. The lesion diameters (severity level) were used in order to calculate the area under the disease progress curve (AUDPC). The inoculation of detached D leaves was the most efficient, fast and inexpensive method, and the one that most satisfactorily reproduced the disease symptoms. The period of 10 to 20 days after inoculation of the D detached leaves with the pathogen is the most suitable to evaluate the resistance of pineapple accessions to fusariosis. The lowest lesion area and AUDPC was found in the accession 1, in all evaluations. Thus, the accession 1 can be used in pineapple breeding programs for resistance to fusariosis.info:eu-repo/semantics/openAccessUniversidade Federal Rural do Semi-ÁridoRevista Caatinga v.30 n.2 20172017-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-21252017000200353en10.1590/1983-21252017v30n210rc
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libraryname SciELO
language English
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author GARCIA,WANDREILLA MOREIRA
KRAUSE,WILLIAN
ARAÚJO,DEJÂNIA VIEIRA DE
KARSBURG,ISANE VERA
DALLACORT,RIVANILDO
spellingShingle GARCIA,WANDREILLA MOREIRA
KRAUSE,WILLIAN
ARAÚJO,DEJÂNIA VIEIRA DE
KARSBURG,ISANE VERA
DALLACORT,RIVANILDO
METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)
author_facet GARCIA,WANDREILLA MOREIRA
KRAUSE,WILLIAN
ARAÚJO,DEJÂNIA VIEIRA DE
KARSBURG,ISANE VERA
DALLACORT,RIVANILDO
author_sort GARCIA,WANDREILLA MOREIRA
title METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)
title_short METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)
title_full METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)
title_fullStr METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)
title_full_unstemmed METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)
title_sort methods for inoculation with fusarium guttiforme and genetic resistance of pineapple (ananas comosus var. comosus)
description ABSTRACT The objective of this work was to evaluate Fusarium guttiforme inoculation methods and genetic resistance of pineapple accessions. Thus, three experiments were conducted: pathogen inoculation of different leaf types (B, D and F) of pineapple (1), pathogen inoculation of pineapple cuttings and detached D leaves (2), and identification of resistance to fusariosis in 19 pineapple accessions (3) sampled in the State of Mato Grosso, Brazil. The cultivars Pérola (susceptible to fusariosis) and BRS-Vitória (resistant to fusariosis) were used as controls. The fusariosis severity was evaluated at 10, 15, 20, 25 and 30 days after inoculation with F. guttiforme. The lesion diameters (severity level) were used in order to calculate the area under the disease progress curve (AUDPC). The inoculation of detached D leaves was the most efficient, fast and inexpensive method, and the one that most satisfactorily reproduced the disease symptoms. The period of 10 to 20 days after inoculation of the D detached leaves with the pathogen is the most suitable to evaluate the resistance of pineapple accessions to fusariosis. The lowest lesion area and AUDPC was found in the accession 1, in all evaluations. Thus, the accession 1 can be used in pineapple breeding programs for resistance to fusariosis.
publisher Universidade Federal Rural do Semi-Árido
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-21252017000200353
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