Determination of inosine 5’-monophosphate (IMP) and guanosine 5’-monophosphate (GMP) in yeast extracts using UV spectroscopy and chemometrics
Abstract This study proposed a method to determine the sum of the concentrations of inosine 5’-monophosphate (IMP) and guanosine 5’-monophosphate (GMP) in final and intermediate products through a combination of UV spectroscopy and multivariate calibration. Partial Least Squares regression (PLS regression) was used for the data analysis and the proposed methods presented errors of around 4 mg/L. In addition, the analysis cost is 10 times lower than the reference chromatographic method and the analytical frequency is 20 samples/hour.
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Format: | Digital revista |
Language: | English |
Published: |
Instituto de Tecnologia de Alimentos - ITAL
2018
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232018000100500 |
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