Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primers

ABSTRACT. With the rise of world fish farming, the national scenario is favorable for using native fish for intensive farming. Among the catfish, the Amazonian Jundiá (Leiarius marmoratus) is a robust candidate, easy to grow and with good organoleptic characteristics in its flesh. For productive success in captivity, it is necessary to consider some questions about the species, such as genetic variability, which must have an acceptable level in a breeding stock, in order to maintain a good diversity; this reduces losses due to inbreeding and low diversity. Therefore, the objective of this study was to characterize the genetic variability of commercial stocks of L. marmoratus from the State of Mato Grosso through microsatellite molecular markers. We analyzed 143 individuals from three stocks. The mean heterozygosity and the inbreeding coefficients observed were 0.060; 0.084; 0.141; and 0.539; 0.562; 0.514, respectively, for the stocks of Campo Verde, Juína, and Nova Mutum. The Deviation in the Hardy-Weinberg equilibrium was observed in most of the loci in the three populations. Considering the genetic differentiation, it is concluded that the three populations are very close genetically, which requires introduction of new genetic material in the stocks to enrich them genetically for a later reproductive program.

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Main Authors: Mantovani,Laís Santana Celestino, Souza,Felipe Pinheiro de, Campos,Eric Costa, Lewandowski,Vanessa, Barrero,Nelson Maurício Lopera, Ribeiro,Ricardo Pereira
Format: Digital revista
Language:English
Published: Editora da Universidade Estadual de Maringá - EDUEM 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86722022000100101
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spelling oai:scielo:S1807-867220220001001012022-02-25Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primersMantovani,Laís Santana CelestinoSouza,Felipe Pinheiro deCampos,Eric CostaLewandowski,VanessaBarrero,Nelson Maurício LoperaRibeiro,Ricardo Pereira DNA molecular markers genetic variability fish culture siluriformes ABSTRACT. With the rise of world fish farming, the national scenario is favorable for using native fish for intensive farming. Among the catfish, the Amazonian Jundiá (Leiarius marmoratus) is a robust candidate, easy to grow and with good organoleptic characteristics in its flesh. For productive success in captivity, it is necessary to consider some questions about the species, such as genetic variability, which must have an acceptable level in a breeding stock, in order to maintain a good diversity; this reduces losses due to inbreeding and low diversity. Therefore, the objective of this study was to characterize the genetic variability of commercial stocks of L. marmoratus from the State of Mato Grosso through microsatellite molecular markers. We analyzed 143 individuals from three stocks. The mean heterozygosity and the inbreeding coefficients observed were 0.060; 0.084; 0.141; and 0.539; 0.562; 0.514, respectively, for the stocks of Campo Verde, Juína, and Nova Mutum. The Deviation in the Hardy-Weinberg equilibrium was observed in most of the loci in the three populations. Considering the genetic differentiation, it is concluded that the three populations are very close genetically, which requires introduction of new genetic material in the stocks to enrich them genetically for a later reproductive program.info:eu-repo/semantics/openAccessEditora da Universidade Estadual de Maringá - EDUEMActa Scientiarum. Animal Sciences v.44 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86722022000100101en10.4025/actascianimsci.v44i1.52657
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countrycode BR
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libraryname SciELO
language English
format Digital
author Mantovani,Laís Santana Celestino
Souza,Felipe Pinheiro de
Campos,Eric Costa
Lewandowski,Vanessa
Barrero,Nelson Maurício Lopera
Ribeiro,Ricardo Pereira
spellingShingle Mantovani,Laís Santana Celestino
Souza,Felipe Pinheiro de
Campos,Eric Costa
Lewandowski,Vanessa
Barrero,Nelson Maurício Lopera
Ribeiro,Ricardo Pereira
Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primers
author_facet Mantovani,Laís Santana Celestino
Souza,Felipe Pinheiro de
Campos,Eric Costa
Lewandowski,Vanessa
Barrero,Nelson Maurício Lopera
Ribeiro,Ricardo Pereira
author_sort Mantovani,Laís Santana Celestino
title Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primers
title_short Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primers
title_full Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primers
title_fullStr Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primers
title_full_unstemmed Genetic diversity in Amazonian Jundiá (Leiarius marmoratus) stocks using heterologous primers
title_sort genetic diversity in amazonian jundiá (leiarius marmoratus) stocks using heterologous primers
description ABSTRACT. With the rise of world fish farming, the national scenario is favorable for using native fish for intensive farming. Among the catfish, the Amazonian Jundiá (Leiarius marmoratus) is a robust candidate, easy to grow and with good organoleptic characteristics in its flesh. For productive success in captivity, it is necessary to consider some questions about the species, such as genetic variability, which must have an acceptable level in a breeding stock, in order to maintain a good diversity; this reduces losses due to inbreeding and low diversity. Therefore, the objective of this study was to characterize the genetic variability of commercial stocks of L. marmoratus from the State of Mato Grosso through microsatellite molecular markers. We analyzed 143 individuals from three stocks. The mean heterozygosity and the inbreeding coefficients observed were 0.060; 0.084; 0.141; and 0.539; 0.562; 0.514, respectively, for the stocks of Campo Verde, Juína, and Nova Mutum. The Deviation in the Hardy-Weinberg equilibrium was observed in most of the loci in the three populations. Considering the genetic differentiation, it is concluded that the three populations are very close genetically, which requires introduction of new genetic material in the stocks to enrich them genetically for a later reproductive program.
publisher Editora da Universidade Estadual de Maringá - EDUEM
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86722022000100101
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