Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats

Abstract Introduction Among the therapeutic effects of ozone therapy, improvement in cell metabolism and peripheral tissue oxygenation have been highlighted. Objective to evaluate the systemic effect of bio-oxidative therapy with ozone gas on wound healing. Material and method Tissue lesions with a circumference of 1.0 cm were induced in the skin on the back of 24 male Wistar rats. The animals were randomly divided into two groups: 1) Group C (control; n=12): with simulation of the application of ozone gas via the rectum and, 2) Group O3 (test; n=12): with application of ozone gas by means of rectal insufflation at a concentration of 50 µg/mL. The animals were euthanized at seven and 15 days, and samples were removed, fixed in formalin, and submitted to macroscopic, histological, and histometric analyses. Result The animals in the O3 group presented mixed inflammation at seven days, which translated into an absence of inflammation at 15 days. The C group exhibited acute inflammation on the 7th day, translating to chronic inflammation, which significantly increased from the 7th to the 15th day. The findings showed that the O3 group presented greater wound contraction (P<0.05) and a greater degree of neovascularization on the 7th day (P<0.05) when compared to group C. On the 15th day, both groups (O3 and C) showed complete re-epithelialization, however, the O3 group demonstrated complete muscle regeneration. Conclusion The systemic ozone therapy had a biomodulatory effect, reducing the characteristics of acute inflammation and increasing tissue repair and regeneration in rat skin.

Saved in:
Bibliographic Details
Main Authors: PIRES,Juliana Rico, KARAM,Alexandre Melo, GARCIA,Valdir Gouveia, RIBEIRO,Fernando Salimon, PONTES,Ana Emilia Farias, ANDRADE,Cleverton Roberto de, ZUZA,Elizangela Cruvinel
Format: Digital revista
Language:English
Published: Universidade Estadual Paulista Júlio de Mesquita Filho 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772021000100415
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S1807-25772021000100415
record_format ojs
spelling oai:scielo:S1807-257720210001004152021-10-26Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in ratsPIRES,Juliana RicoKARAM,Alexandre MeloGARCIA,Valdir GouveiaRIBEIRO,Fernando SalimonPONTES,Ana Emilia FariasANDRADE,Cleverton Roberto deZUZA,Elizangela Cruvinel Ozone wound healing inflammation rats Abstract Introduction Among the therapeutic effects of ozone therapy, improvement in cell metabolism and peripheral tissue oxygenation have been highlighted. Objective to evaluate the systemic effect of bio-oxidative therapy with ozone gas on wound healing. Material and method Tissue lesions with a circumference of 1.0 cm were induced in the skin on the back of 24 male Wistar rats. The animals were randomly divided into two groups: 1) Group C (control; n=12): with simulation of the application of ozone gas via the rectum and, 2) Group O3 (test; n=12): with application of ozone gas by means of rectal insufflation at a concentration of 50 µg/mL. The animals were euthanized at seven and 15 days, and samples were removed, fixed in formalin, and submitted to macroscopic, histological, and histometric analyses. Result The animals in the O3 group presented mixed inflammation at seven days, which translated into an absence of inflammation at 15 days. The C group exhibited acute inflammation on the 7th day, translating to chronic inflammation, which significantly increased from the 7th to the 15th day. The findings showed that the O3 group presented greater wound contraction (P<0.05) and a greater degree of neovascularization on the 7th day (P<0.05) when compared to group C. On the 15th day, both groups (O3 and C) showed complete re-epithelialization, however, the O3 group demonstrated complete muscle regeneration. Conclusion The systemic ozone therapy had a biomodulatory effect, reducing the characteristics of acute inflammation and increasing tissue repair and regeneration in rat skin.info:eu-repo/semantics/openAccessUniversidade Estadual Paulista Júlio de Mesquita FilhoRevista de Odontologia da UNESP v.50 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772021000100415en10.1590/1807-2577.04621
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author PIRES,Juliana Rico
KARAM,Alexandre Melo
GARCIA,Valdir Gouveia
RIBEIRO,Fernando Salimon
PONTES,Ana Emilia Farias
ANDRADE,Cleverton Roberto de
ZUZA,Elizangela Cruvinel
spellingShingle PIRES,Juliana Rico
KARAM,Alexandre Melo
GARCIA,Valdir Gouveia
RIBEIRO,Fernando Salimon
PONTES,Ana Emilia Farias
ANDRADE,Cleverton Roberto de
ZUZA,Elizangela Cruvinel
Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats
author_facet PIRES,Juliana Rico
KARAM,Alexandre Melo
GARCIA,Valdir Gouveia
RIBEIRO,Fernando Salimon
PONTES,Ana Emilia Farias
ANDRADE,Cleverton Roberto de
ZUZA,Elizangela Cruvinel
author_sort PIRES,Juliana Rico
title Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats
title_short Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats
title_full Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats
title_fullStr Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats
title_full_unstemmed Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats
title_sort effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats
description Abstract Introduction Among the therapeutic effects of ozone therapy, improvement in cell metabolism and peripheral tissue oxygenation have been highlighted. Objective to evaluate the systemic effect of bio-oxidative therapy with ozone gas on wound healing. Material and method Tissue lesions with a circumference of 1.0 cm were induced in the skin on the back of 24 male Wistar rats. The animals were randomly divided into two groups: 1) Group C (control; n=12): with simulation of the application of ozone gas via the rectum and, 2) Group O3 (test; n=12): with application of ozone gas by means of rectal insufflation at a concentration of 50 µg/mL. The animals were euthanized at seven and 15 days, and samples were removed, fixed in formalin, and submitted to macroscopic, histological, and histometric analyses. Result The animals in the O3 group presented mixed inflammation at seven days, which translated into an absence of inflammation at 15 days. The C group exhibited acute inflammation on the 7th day, translating to chronic inflammation, which significantly increased from the 7th to the 15th day. The findings showed that the O3 group presented greater wound contraction (P<0.05) and a greater degree of neovascularization on the 7th day (P<0.05) when compared to group C. On the 15th day, both groups (O3 and C) showed complete re-epithelialization, however, the O3 group demonstrated complete muscle regeneration. Conclusion The systemic ozone therapy had a biomodulatory effect, reducing the characteristics of acute inflammation and increasing tissue repair and regeneration in rat skin.
publisher Universidade Estadual Paulista Júlio de Mesquita Filho
publishDate 2021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772021000100415
work_keys_str_mv AT piresjulianarico effectofsystemicozonetherapyasabiomodulatoroftissueregenerationandinflammatoryresponseinrats
AT karamalexandremelo effectofsystemicozonetherapyasabiomodulatoroftissueregenerationandinflammatoryresponseinrats
AT garciavaldirgouveia effectofsystemicozonetherapyasabiomodulatoroftissueregenerationandinflammatoryresponseinrats
AT ribeirofernandosalimon effectofsystemicozonetherapyasabiomodulatoroftissueregenerationandinflammatoryresponseinrats
AT pontesanaemiliafarias effectofsystemicozonetherapyasabiomodulatoroftissueregenerationandinflammatoryresponseinrats
AT andradeclevertonrobertode effectofsystemicozonetherapyasabiomodulatoroftissueregenerationandinflammatoryresponseinrats
AT zuzaelizangelacruvinel effectofsystemicozonetherapyasabiomodulatoroftissueregenerationandinflammatoryresponseinrats
_version_ 1756431894890676224