Effect of enhanced masticatory force on OPG, RANKL and MGF in alveolar bone of ovariectomized rats
Abstract Menopause induces oral bone loss, leading to various oral diseases. Mastication importantly affects bone metabolism in the jawbone. Objective: To analyze the effect of enhanced masticatory force on osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), and mechano–growth factor (MGF) in alveolar bone of ovariectomized rats and to study the mechanics mechanism of the alveolar bone of ovariectomized rats response to enhanced masticatory force. Methodology: Thirty Sprague Dawley rats were randomly divided into three groups: sham–operation group (fat around the removed ovary + normal hard diet), model group (ovariectomy + normal hard diet), and experimental group (ovariectomy + high hard diet). It was a 2–month experiment. Enzyme–linked immunosorbent assay (ELISA) detected serum estradiol (E2), osteocalcin (BGP) and alkaline phosphatase (ALP) in rats. Bone histomorphometric indices in the third molar region of maxilla were detected by micro-CT; protein expressions of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Western blot; and gene expression of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Quantitative Real–Time PCR. Results: Comparing with model group, serum E2 in experimental group increased but not significantly, serum BGP and serum ALP in experimental group decreased but not significantly, OPG in experimental group in alveolar bone increased significantly, RANKL in experimental group in alveolar bone decreased significantly, RANKL/OPG ratio in experimental group decreased significantly, MGF in experimental group in alveolar bone increased significantly, bone volume to total volume fraction increased significantly in experimental group, trabecular thickness increased significantly in experimental group, and trabecular separation decreased significantly in experimental group. Conclusion: Enhanced masticatory force affected the expression of OPG, RANKL, and MGF in alveolar bone of ovariectomized rats, improved the quality of jaw bone of ovariectomized rats, and delayed oral bone loss by ovariectomy.
Main Authors: | Ma,Zongmin, Li,Shuxian, Sun,Yuchen |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Faculdade De Odontologia De Bauru - USP
2020
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572020000100431 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Effect of sumac extract on serum oxidative status, RANKL/OPG system and alveolar bone loss in experimental periodontitis in rats
by: SAĞLAM,Mehmet, et al.
Published: (2015) -
The RANK/ RANKL/ OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency
by: TERA,Tábata de Mello, et al.
Published: (2014) -
The effects of chitosan oligosaccharides on OPG and RANKL expression in a rat osteoarthritis model
by: Zhang,Chun, et al.
Published: (2017) -
Sheep bone collagen peptide ameliorates osteoporosis by regulating RANK/RANKL/OPG signal pathway
by: ZHU,Yixin, et al.
Published: (2022) -
RANK/RANKL/OPG Expression in Rapid Maxillary Expansion
by: Arnez,Maya Fernanda Manfrin, et al.
Published: (2017)