Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients

Abstract Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of this virus are vital for medical decision-making. Identification of SARS-CoV-2 infection cases is based on detection of the virus RNA by molecular tests, particularly real-time reverse transcription-polymerase chain reaction (RT-PCR). Technical and operational details specific to each center must be considered to perform the molecular diagnosis of SARS-CoV-2 in pediatric patients. The term “qualified laboratories” involves laboratories in which all users, analysts, and anyone reporting results are trained to develop and interpret results through a procedure implemented previously by an instructor. Such knowledge is essential in detecting and identifying errors during each of its phases: pre-analytical, analytical, and post-analytical, which allow the establishment of continuous improvement policies to ensure the quality of the results, but above all, the physical integrity of health workers.

Bibliographic Details

Main Authors:	Parra-Ortega,Israel, Vilchis-Ordoñez,Armando, López-Martínez,Briceida, Angeles-Floriano,Tania
Format:	Digital revista
Language:	English
Published:	Instituto Nacional de Salud, Hospital Infantil de México Federico Gómez 2021
Online Access:	http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1665-11462021000300171
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