Polymorphism C242T in the Cyp19 gene in meat sheep
Abstract The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423).
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Instituto Internacional de Ecologia
2016
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oai:scielo:S1519-698420160001002052016-02-19Polymorphism C242T in the Cyp19 gene in meat sheepMora,N. H. A. P.Silva,S. C. C.Tanamati,F.Schuroff,G. P.Macedo,F. A. F.Gasparino,E. Cyp19 Dorper polymorphism Santa Inês Texel White Dorper Abstract The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423).info:eu-repo/semantics/openAccessInstituto Internacional de EcologiaBrazilian Journal of Biology v.76 n.1 20162016-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842016000100205en10.1590/1519-6984.17414 |
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| author |
Mora,N. H. A. P. Silva,S. C. C. Tanamati,F. Schuroff,G. P. Macedo,F. A. F. Gasparino,E. |
| spellingShingle |
Mora,N. H. A. P. Silva,S. C. C. Tanamati,F. Schuroff,G. P. Macedo,F. A. F. Gasparino,E. Polymorphism C242T in the Cyp19 gene in meat sheep |
| author_facet |
Mora,N. H. A. P. Silva,S. C. C. Tanamati,F. Schuroff,G. P. Macedo,F. A. F. Gasparino,E. |
| author_sort |
Mora,N. H. A. P. |
| title |
Polymorphism C242T in the Cyp19 gene in meat sheep |
| title_short |
Polymorphism C242T in the Cyp19 gene in meat sheep |
| title_full |
Polymorphism C242T in the Cyp19 gene in meat sheep |
| title_fullStr |
Polymorphism C242T in the Cyp19 gene in meat sheep |
| title_full_unstemmed |
Polymorphism C242T in the Cyp19 gene in meat sheep |
| title_sort |
polymorphism c242t in the cyp19 gene in meat sheep |
| description |
Abstract The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423). |
| publisher |
Instituto Internacional de Ecologia |
| publishDate |
2016 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842016000100205 |
| work_keys_str_mv |
AT moranhap polymorphismc242tinthecyp19geneinmeatsheep AT silvascc polymorphismc242tinthecyp19geneinmeatsheep AT tanamatif polymorphismc242tinthecyp19geneinmeatsheep AT schuroffgp polymorphismc242tinthecyp19geneinmeatsheep AT macedofaf polymorphismc242tinthecyp19geneinmeatsheep AT gasparinoe polymorphismc242tinthecyp19geneinmeatsheep |
| _version_ |
1756426624877723648 |