Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality

Abstract Objectives: microbiological quality of raw human milk (HM) from donors at the HMB (Human Milk Bank) of Santa Casa de Misericórdia de Ouro Preto was evaluated. Methods: the microbiological quality of milk from each of 10 donors in triplicate (30 samples) was evaluated by enumeration of Staphylococcus aureus and total aerobic mesophiles (surface plating) and total and thermotolerant coliforms (Most Probable Number - MPN). A questionnaire and a checklist were applied after the end of sample collection to verify the compliance of milk extraction and storage procedures by the donors. Results: inadequacies in the procedures for expression and storage of donated HM were observed. The presence of all microorganisms studied were verified in the milk of all donors, and in some cases exceeded the safety limits. The checklist indicated that some necessary hygienic-sanitary procedures were not carefully performed, making it important to properly guide the donors. Conclusions: this study points to an unfavorable scenario for the safety and quality of donated HM, and it is important to encourage further studies to be conducted so that the Brazilian network of HMB adopts stricter criteria for instructions on procedures and support for HM donors.

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Main Authors: Chaves,Jaísa Oliveira, Fernandes,Angélica Maria de Freitas, Parreiras,Paola Machado, Passos,Maria Cristina, Cunha,Luciana Rodrigues da, Menezes,Camila Carvalho
Format: Digital revista
Language:English
Published: Instituto de Medicina Integral Prof. Fernando Figueira 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-38292022000400863
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spelling oai:scielo:S1519-382920220004008632023-01-24Compliance in handling of donated raw breast milk to human milk banks regarding microbiological qualityChaves,Jaísa OliveiraFernandes,Angélica Maria de FreitasParreiras,Paola MachadoPassos,Maria CristinaCunha,Luciana Rodrigues daMenezes,Camila Carvalho Donor Food microbiology Human milk Milk banks Quality control Abstract Objectives: microbiological quality of raw human milk (HM) from donors at the HMB (Human Milk Bank) of Santa Casa de Misericórdia de Ouro Preto was evaluated. Methods: the microbiological quality of milk from each of 10 donors in triplicate (30 samples) was evaluated by enumeration of Staphylococcus aureus and total aerobic mesophiles (surface plating) and total and thermotolerant coliforms (Most Probable Number - MPN). A questionnaire and a checklist were applied after the end of sample collection to verify the compliance of milk extraction and storage procedures by the donors. Results: inadequacies in the procedures for expression and storage of donated HM were observed. The presence of all microorganisms studied were verified in the milk of all donors, and in some cases exceeded the safety limits. The checklist indicated that some necessary hygienic-sanitary procedures were not carefully performed, making it important to properly guide the donors. Conclusions: this study points to an unfavorable scenario for the safety and quality of donated HM, and it is important to encourage further studies to be conducted so that the Brazilian network of HMB adopts stricter criteria for instructions on procedures and support for HM donors.info:eu-repo/semantics/openAccessInstituto de Medicina Integral Prof. Fernando FigueiraRevista Brasileira de Saúde Materno Infantil v.22 n.4 20222022-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-38292022000400863en10.1590/1806-9304202200040008
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country Brasil
countrycode BR
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Chaves,Jaísa Oliveira
Fernandes,Angélica Maria de Freitas
Parreiras,Paola Machado
Passos,Maria Cristina
Cunha,Luciana Rodrigues da
Menezes,Camila Carvalho
spellingShingle Chaves,Jaísa Oliveira
Fernandes,Angélica Maria de Freitas
Parreiras,Paola Machado
Passos,Maria Cristina
Cunha,Luciana Rodrigues da
Menezes,Camila Carvalho
Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality
author_facet Chaves,Jaísa Oliveira
Fernandes,Angélica Maria de Freitas
Parreiras,Paola Machado
Passos,Maria Cristina
Cunha,Luciana Rodrigues da
Menezes,Camila Carvalho
author_sort Chaves,Jaísa Oliveira
title Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality
title_short Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality
title_full Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality
title_fullStr Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality
title_full_unstemmed Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality
title_sort compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality
description Abstract Objectives: microbiological quality of raw human milk (HM) from donors at the HMB (Human Milk Bank) of Santa Casa de Misericórdia de Ouro Preto was evaluated. Methods: the microbiological quality of milk from each of 10 donors in triplicate (30 samples) was evaluated by enumeration of Staphylococcus aureus and total aerobic mesophiles (surface plating) and total and thermotolerant coliforms (Most Probable Number - MPN). A questionnaire and a checklist were applied after the end of sample collection to verify the compliance of milk extraction and storage procedures by the donors. Results: inadequacies in the procedures for expression and storage of donated HM were observed. The presence of all microorganisms studied were verified in the milk of all donors, and in some cases exceeded the safety limits. The checklist indicated that some necessary hygienic-sanitary procedures were not carefully performed, making it important to properly guide the donors. Conclusions: this study points to an unfavorable scenario for the safety and quality of donated HM, and it is important to encourage further studies to be conducted so that the Brazilian network of HMB adopts stricter criteria for instructions on procedures and support for HM donors.
publisher Instituto de Medicina Integral Prof. Fernando Figueira
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-38292022000400863
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