Comparison between ectodomain and G2 region of G glycoprotein for genotyping of HRSV

The Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis of G protein and a comparative study between G2 region and ectodomain of attachment glycoprotein. Fifty-three nasal swab samples from children less than 5 years old and presenting symptoms of acute respiratory illness, assisted at the University Hospital (UH) of University of Sao Paulo (USP) in 2004, were submitted to sequencing by PCR and compared with GenBank sequences. We concluded that the G2 region is adequate for HRSV genotyping.

Bibliographic Details

Main Authors:	Campos,Angélica Cristine de Almeida, Durigon,Edison Luiz, Leal,Andréa Lima, Silva,Tereza Souza, Bosso,Patrícia Alves Ramos, Moraes,Claudia Trigo Pedroso, Oliveira,Danielle Bruna Leal, Lima,Hildener Nogueira, Vieira,Sandra Elisabete, Botosso,Viviane Fongaro, Zanotto,Paolo Marinho de Andrade, Stewien,Klaus Eberhard
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Microbiologia 2007
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300005
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