Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women

Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.

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Main Authors: Oliveira,Jéssica de, Tureck,Luciane Viater, Santos,Willian dos, Saliba,Louise Farah, Schenknecht,Caroline Schovanz, Scaraboto,Débora, Souza,Ricardo Lehtonen R., Furtado-Alle,Lupe
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Genética 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408
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spelling oai:scielo:S1415-475720170003004082017-06-28Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in womenOliveira,Jéssica deTureck,Luciane ViaterSantos,Willian dosSaliba,Louise FarahSchenknecht,Caroline SchovanzScaraboto,DéboraSouza,Ricardo Lehtonen R.Furtado-Alle,Lupe BCHE gene obesity lipid metabolism polymorphisms Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.40 n.2 20172017-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408en10.1590/1678-4685-gmb-2016-0123
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country Brasil
countrycode BR
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libraryname SciELO
language English
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author Oliveira,Jéssica de
Tureck,Luciane Viater
Santos,Willian dos
Saliba,Louise Farah
Schenknecht,Caroline Schovanz
Scaraboto,Débora
Souza,Ricardo Lehtonen R.
Furtado-Alle,Lupe
spellingShingle Oliveira,Jéssica de
Tureck,Luciane Viater
Santos,Willian dos
Saliba,Louise Farah
Schenknecht,Caroline Schovanz
Scaraboto,Débora
Souza,Ricardo Lehtonen R.
Furtado-Alle,Lupe
Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
author_facet Oliveira,Jéssica de
Tureck,Luciane Viater
Santos,Willian dos
Saliba,Louise Farah
Schenknecht,Caroline Schovanz
Scaraboto,Débora
Souza,Ricardo Lehtonen R.
Furtado-Alle,Lupe
author_sort Oliveira,Jéssica de
title Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_short Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_full Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_fullStr Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_full_unstemmed Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
title_sort effect of bche single nucleotide polymorphisms on lipid metabolism markers in women
description Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.
publisher Sociedade Brasileira de Genética
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408
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