Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.
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Sociedade Brasileira de Genética
2017
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oai:scielo:S1415-475720170003004082017-06-28Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in womenOliveira,Jéssica deTureck,Luciane ViaterSantos,Willian dosSaliba,Louise FarahSchenknecht,Caroline SchovanzScaraboto,DéboraSouza,Ricardo Lehtonen R.Furtado-Alle,Lupe BCHE gene obesity lipid metabolism polymorphisms Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.40 n.2 20172017-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408en10.1590/1678-4685-gmb-2016-0123 |
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Oliveira,Jéssica de Tureck,Luciane Viater Santos,Willian dos Saliba,Louise Farah Schenknecht,Caroline Schovanz Scaraboto,Débora Souza,Ricardo Lehtonen R. Furtado-Alle,Lupe |
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Oliveira,Jéssica de Tureck,Luciane Viater Santos,Willian dos Saliba,Louise Farah Schenknecht,Caroline Schovanz Scaraboto,Débora Souza,Ricardo Lehtonen R. Furtado-Alle,Lupe Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women |
author_facet |
Oliveira,Jéssica de Tureck,Luciane Viater Santos,Willian dos Saliba,Louise Farah Schenknecht,Caroline Schovanz Scaraboto,Débora Souza,Ricardo Lehtonen R. Furtado-Alle,Lupe |
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Oliveira,Jéssica de |
title |
Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women |
title_short |
Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women |
title_full |
Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women |
title_fullStr |
Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women |
title_full_unstemmed |
Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women |
title_sort |
effect of bche single nucleotide polymorphisms on lipid metabolism markers in women |
description |
Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms. |
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Sociedade Brasileira de Genética |
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2017 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408 |
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