A shortcut in phage screening technique

A simple modification of the traditional Benton & Davis technique for phage screening is presented that avoids the tedious sample dilutions of putative spots/phages towards the second screening. With the use of a sole agar plate and nylon filter, the modification distinguishes a true positive recombinant from a false positive, with high probability of success.

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Bibliographic Details
Main Authors: Andrade,Alexandre de, Teixeira,Paula Rezende, Siviero,Fábio, Santelli,Roberto Vicente
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Genética 2005
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100025
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