A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli

A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli

OBJECTIVES: Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells.METHODS: In the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were analyzed by ELISA and Western blotting. Efficiency of the immune sera against ETEC was evaluated.RESULTS: Antibody induction was followed by immunization of mice with the chimeric protein. Pretreatment of the ETEC cells with immunized animal antisera remarkably decreased their adhesion to Caco-2 cells.DISCUSSION: The results indicate efficacy of the recombinant chimeric protein as an effective immunogen, which induces strong humoral response as well as protection against ETEC adherence and toxicity.

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Main Authors: Bagheri,Samane, Gargari,Seyed Latif Mousavi, Rasooli,Iraj, Nazarian,Shahram, Alerasol,Masoome
Format: Digital revista
Language:English
Published: Brazilian Society of Infectious Diseases 2014
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300308
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spelling oai:scielo:S1413-867020140003003082015-10-26A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coliBagheri,SamaneGargari,Seyed Latif MousaviRasooli,IrajNazarian,ShahramAlerasol,Masoome Enterotoxigenic Escherichia coli CS6 Enterotoxin Chimeric protein OBJECTIVES: Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells.METHODS: In the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were analyzed by ELISA and Western blotting. Efficiency of the immune sera against ETEC was evaluated.RESULTS: Antibody induction was followed by immunization of mice with the chimeric protein. Pretreatment of the ETEC cells with immunized animal antisera remarkably decreased their adhesion to Caco-2 cells.DISCUSSION: The results indicate efficacy of the recombinant chimeric protein as an effective immunogen, which induces strong humoral response as well as protection against ETEC adherence and toxicity.info:eu-repo/semantics/openAccessBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious Diseases v.18 n.3 20142014-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300308en10.1016/j.bjid.2013.07.012
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Bagheri,Samane
Gargari,Seyed Latif Mousavi
Rasooli,Iraj
Nazarian,Shahram
Alerasol,Masoome
spellingShingle Bagheri,Samane
Gargari,Seyed Latif Mousavi
Rasooli,Iraj
Nazarian,Shahram
Alerasol,Masoome
A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
author_facet Bagheri,Samane
Gargari,Seyed Latif Mousavi
Rasooli,Iraj
Nazarian,Shahram
Alerasol,Masoome
author_sort Bagheri,Samane
title A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
title_short A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
title_full A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
title_fullStr A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
title_full_unstemmed A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
title_sort cssa, cssb and ltb chimeric protein induces protection against enterotoxigenic escherichia coli
description OBJECTIVES: Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells.METHODS: In the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were analyzed by ELISA and Western blotting. Efficiency of the immune sera against ETEC was evaluated.RESULTS: Antibody induction was followed by immunization of mice with the chimeric protein. Pretreatment of the ETEC cells with immunized animal antisera remarkably decreased their adhesion to Caco-2 cells.DISCUSSION: The results indicate efficacy of the recombinant chimeric protein as an effective immunogen, which induces strong humoral response as well as protection against ETEC adherence and toxicity.
publisher Brazilian Society of Infectious Diseases
publishDate 2014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300308
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