ULTRASOUND-ASSISTED EXTRACTION AND CHARACTERIZATION OF POLYSACCHARIDE FROM MARYLAND TOBACCO LEAVES

Ultrasound-assisted extraction (UAE) of polysaccharide from Maryland tobacco leaves was studied by response surface methodology. Furthermore, the crude polysaccharide was purified and two components (Fr-I and Fr-II) were obtained. FT-IR spectral analysis of the purified polysaccharide revealed prominent characteristic groups. The monosaccharide composition analysis by gas chromatography-mass spectrometry (GC/MS) indicated the main composition between Fr-I and Fr-II was different. Furthermore, thermo gravimetric analysis (TGA) indicated the degradation temperature (Td) of the Fr-I (241 ºC) was higher than those of Fr-II (216 ºC). Detected by the pyrolysis gas chromatography-mass spectrometry (py-GC/MS), it was found that the main kinds of pyrolysis products from both Fr-I and Fr-II were similar. Finally, On the basis of hydroxyl and DPPH radical scavenging assay, Fr-II has stronger antioxidant activities than Fr-I. The thermal behavior and antioxidant activity might be attributed to the configuration of the chemical compositions.

Bibliographic Details

Main Authors:	LIU,SHAOHUA, HE,PEIXIN, TIAN,ZHAOFU, LI,XIAOLAN, XU,CHUNPING
Format:	Digital revista
Language:	English
Published:	Sociedad Chilena de Química 2015
Online Access:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000100001
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