Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model
SUMMARY: Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavernosum of rats submitted to a semivoluntary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression of AIF and PARP and their regulatory microRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoluntary alcoholism.
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Sociedad Chilena de Anatomía
2020
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oai:scielo:S0717-950220200006016392020-10-30Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism ModelSarraipo,Vagner SchiavoniLizarte Neto,Fermino SanchesCarvalho,Camila Albuquerque Mello deSilva,Jairo Pinheiro daDurand,Marina ToledoSchimming,Bruno CezarMartinez,MarceloGalli,Maria de Fátima Tazima SoritaBuono,Fabianna de OliveiraTiezzi,Maria Fernanda Barbirato da MataNovais,Paulo CezarGula,Isabella StracieriThomazini,José AntônioFazan,Valeria Paula SassoliRodrigues,Andressa RomualdoTirapelli,Daniela Pretti da CunhaCirino,Mucio Luiz de AssisMolina,Carlos Augusto FernandesJunior,Silvio TucciTirapelli,Luis Fernando Alcoholism Apoptosis Erectile Dysfunction MicroRNAs SUMMARY: Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavernosum of rats submitted to a semivoluntary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression of AIF and PARP and their regulatory microRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoluntary alcoholism.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.6 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601639en10.4067/S0717-95022020000601639 |
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Sarraipo,Vagner Schiavoni Lizarte Neto,Fermino Sanches Carvalho,Camila Albuquerque Mello de Silva,Jairo Pinheiro da Durand,Marina Toledo Schimming,Bruno Cezar Martinez,Marcelo Galli,Maria de Fátima Tazima Sorita Buono,Fabianna de Oliveira Tiezzi,Maria Fernanda Barbirato da Mata Novais,Paulo Cezar Gula,Isabella Stracieri Thomazini,José Antônio Fazan,Valeria Paula Sassoli Rodrigues,Andressa Romualdo Tirapelli,Daniela Pretti da Cunha Cirino,Mucio Luiz de Assis Molina,Carlos Augusto Fernandes Junior,Silvio Tucci Tirapelli,Luis Fernando |
| spellingShingle |
Sarraipo,Vagner Schiavoni Lizarte Neto,Fermino Sanches Carvalho,Camila Albuquerque Mello de Silva,Jairo Pinheiro da Durand,Marina Toledo Schimming,Bruno Cezar Martinez,Marcelo Galli,Maria de Fátima Tazima Sorita Buono,Fabianna de Oliveira Tiezzi,Maria Fernanda Barbirato da Mata Novais,Paulo Cezar Gula,Isabella Stracieri Thomazini,José Antônio Fazan,Valeria Paula Sassoli Rodrigues,Andressa Romualdo Tirapelli,Daniela Pretti da Cunha Cirino,Mucio Luiz de Assis Molina,Carlos Augusto Fernandes Junior,Silvio Tucci Tirapelli,Luis Fernando Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model |
| author_facet |
Sarraipo,Vagner Schiavoni Lizarte Neto,Fermino Sanches Carvalho,Camila Albuquerque Mello de Silva,Jairo Pinheiro da Durand,Marina Toledo Schimming,Bruno Cezar Martinez,Marcelo Galli,Maria de Fátima Tazima Sorita Buono,Fabianna de Oliveira Tiezzi,Maria Fernanda Barbirato da Mata Novais,Paulo Cezar Gula,Isabella Stracieri Thomazini,José Antônio Fazan,Valeria Paula Sassoli Rodrigues,Andressa Romualdo Tirapelli,Daniela Pretti da Cunha Cirino,Mucio Luiz de Assis Molina,Carlos Augusto Fernandes Junior,Silvio Tucci Tirapelli,Luis Fernando |
| author_sort |
Sarraipo,Vagner Schiavoni |
| title |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model |
| title_short |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model |
| title_full |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model |
| title_fullStr |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model |
| title_full_unstemmed |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum Of Rats Subjected to Chronic Alcoholism Model |
| title_sort |
expression of aif, parp, and mirnas mir-145, mir-210, and mir-486 associated with apoptosis in the corpus cavernosum of rats subjected to chronic alcoholism model |
| description |
SUMMARY: Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavernosum of rats submitted to a semivoluntary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression of AIF and PARP and their regulatory microRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoluntary alcoholism. |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2020 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000601639 |
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1755993225957474304 |