LaNe RAGE: a new tool for genomic DNA flanking sequence determination

LaNe RAGE: a new tool for genomic DNA flanking sequence determination

The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient ‘one-sided' PCR mechanisms. I demonstrate the application of a simple ‘two-sided' PCR-based approach, lariat-dependent nested PCR for rapid amplification of genomic DNA ends (LaNe RAGE), applied to the mouse GAPDH and PGK1 gene flanking sequences. This demonstration offers great promise in applications such as genome walking, transposon mutagenesis mapping and DNA fingerprinting

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Bibliographic Details
Main Author: Park,Daniel J
Format: Digital revista
Language:English
Published: Pontificia Universidad Católica de Valparaíso 2005
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200012
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spelling oai:scielo:S0717-345820050002000122005-10-14LaNe RAGE: a new tool for genomic DNA flanking sequence determinationPark,Daniel J cloning DNA fingerprinting genomic DNA walking PCR sequencing transposon mutagenesis The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient ‘one-sided' PCR mechanisms. I demonstrate the application of a simple ‘two-sided' PCR-based approach, lariat-dependent nested PCR for rapid amplification of genomic DNA ends (LaNe RAGE), applied to the mouse GAPDH and PGK1 gene flanking sequences. This demonstration offers great promise in applications such as genome walking, transposon mutagenesis mapping and DNA fingerprintinginfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.2 20052005-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200012en
institution SCIELO
collection OJS
country Chile
countrycode CL
component Revista
access En linea
databasecode rev-scielo-cl
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Park,Daniel J
spellingShingle Park,Daniel J
LaNe RAGE: a new tool for genomic DNA flanking sequence determination
author_facet Park,Daniel J
author_sort Park,Daniel J
title LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_short LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_full LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_fullStr LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_full_unstemmed LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_sort lane rage: a new tool for genomic dna flanking sequence determination
description The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient ‘one-sided' PCR mechanisms. I demonstrate the application of a simple ‘two-sided' PCR-based approach, lariat-dependent nested PCR for rapid amplification of genomic DNA ends (LaNe RAGE), applied to the mouse GAPDH and PGK1 gene flanking sequences. This demonstration offers great promise in applications such as genome walking, transposon mutagenesis mapping and DNA fingerprinting
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2005
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200012
work_keys_str_mv AT parkdanielj lanerageanewtoolforgenomicdnaflankingsequencedetermination
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