Importance of the extraction method in the quantification of total phenolic compounds in Phaseolus Vulgaris L
In order to study the effects of the type of solvent and extraction technique on the quantification of the total phenolic compounds in samples of Phaseolus vulgaris, four techniques of methanol extraction (plate stirring, wrist-action shaking, sonication and homogenization-sonication) and two procedures of sequential extraction, were evaluated. With the various techniques of methanol extraction, significantly different results were obtained. The highest content of total phenolic compounds was obtained with plate stirring. Comparing the six tested procedures, the highest concentrations of total phenolic compounds were obtained with the sequential extraction in water-NaOH 0.2N-methanol. The results showed that the extraction of phenolic compounds of P. vulgaris depends to a large extent on the analytic technique and that the type of solvent used, and the methanol extraction-alkaline hydrolysis-ethylacetate extraction is the method that enables the best quantification. Thus, its use can be recommended for analytical purposes.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
ASOCIACIÓN INTERCIENCIA
2009
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| Online Access: | http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0378-18442009000900011 |
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