Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy

Abstract: The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.

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Bibliographic Details
Main Authors: Coelho,Polyana Galvão Bernardes, Souza,Maria Verônica de, Conceição,Lissandro Gonçalves, Viloria,Marlene Isabel Vargas, Bedoya,Sirley Adriana Ortiz
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Dermatologia 2018
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962018000300415
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