Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens
ABSTRACT: Protozoa of the genus Phytomonas are harmful parasites to several agricultural crops of economic importance. Due to their recognized biological activity, crude extracts of Piper aduncum, P. crassinervium, P. hispidum, and P. amalago leaves, were tested using the microdilution plate technique to assess the antiparasitic potential against Phytomonas serpens. Results showed that the ethanolic crude extract of P. crassinervium and P. amalago presented the best inhibitory concentration for 50% of the cells (IC50), 16.5 µg mL-1 in chloroform phase, and 18 µg mL-1 in aqueous phase, respectively, after 48 h treatment. Cytotoxicity analyses were performed using the colorimetric method of sulforhodamine-B in LLCMK2 mammalian cells. The chloroform phase of P. crassinervium was subjected to the fractionation process, in which the ethyl acetate and dichloromethane fractions obtained better IC50 values. Scanning electron microscopy (SEM) images showed alterations in the cell membrane of the treated parasites. The data obtained indicate a potential antiparasitic effect of the Piper species analyzed against P. serpens, being considered promising candidates for formulations of bioproducts to control the parasite.
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Universidade Federal de Santa Maria
2020
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oai:scielo:S0103-847820200010001522020-08-26Activity of Piperaceae extracts and fractions in the control of Phytomonas serpensCancini,Neriani de SouzaBeraldo-Borrazzo,JesieliMenezes,Jéssica Lima deCortez,Diógenes Aparício GarciaValdez,Rodrigo HinojosaGarcia,Francielle PelegrinYamada-Ogatta,Sueli FumieDias Filho,Benedito PradoUeda-Nakamura,TâniaNakamura,Celso VataruAbreu Filho,Benício Alves de antiparasitic activity medicinal plants Piper Trypanosomatid Phytomonas ABSTRACT: Protozoa of the genus Phytomonas are harmful parasites to several agricultural crops of economic importance. Due to their recognized biological activity, crude extracts of Piper aduncum, P. crassinervium, P. hispidum, and P. amalago leaves, were tested using the microdilution plate technique to assess the antiparasitic potential against Phytomonas serpens. Results showed that the ethanolic crude extract of P. crassinervium and P. amalago presented the best inhibitory concentration for 50% of the cells (IC50), 16.5 µg mL-1 in chloroform phase, and 18 µg mL-1 in aqueous phase, respectively, after 48 h treatment. Cytotoxicity analyses were performed using the colorimetric method of sulforhodamine-B in LLCMK2 mammalian cells. The chloroform phase of P. crassinervium was subjected to the fractionation process, in which the ethyl acetate and dichloromethane fractions obtained better IC50 values. Scanning electron microscopy (SEM) images showed alterations in the cell membrane of the treated parasites. The data obtained indicate a potential antiparasitic effect of the Piper species analyzed against P. serpens, being considered promising candidates for formulations of bioproducts to control the parasite.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.50 n.10 20202020-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001000152en10.1590/0103-8478cr20200343 |
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Cancini,Neriani de Souza Beraldo-Borrazzo,Jesieli Menezes,Jéssica Lima de Cortez,Diógenes Aparício Garcia Valdez,Rodrigo Hinojosa Garcia,Francielle Pelegrin Yamada-Ogatta,Sueli Fumie Dias Filho,Benedito Prado Ueda-Nakamura,Tânia Nakamura,Celso Vataru Abreu Filho,Benício Alves de |
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Cancini,Neriani de Souza Beraldo-Borrazzo,Jesieli Menezes,Jéssica Lima de Cortez,Diógenes Aparício Garcia Valdez,Rodrigo Hinojosa Garcia,Francielle Pelegrin Yamada-Ogatta,Sueli Fumie Dias Filho,Benedito Prado Ueda-Nakamura,Tânia Nakamura,Celso Vataru Abreu Filho,Benício Alves de Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
author_facet |
Cancini,Neriani de Souza Beraldo-Borrazzo,Jesieli Menezes,Jéssica Lima de Cortez,Diógenes Aparício Garcia Valdez,Rodrigo Hinojosa Garcia,Francielle Pelegrin Yamada-Ogatta,Sueli Fumie Dias Filho,Benedito Prado Ueda-Nakamura,Tânia Nakamura,Celso Vataru Abreu Filho,Benício Alves de |
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Cancini,Neriani de Souza |
title |
Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
title_short |
Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
title_full |
Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
title_fullStr |
Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
title_full_unstemmed |
Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
title_sort |
activity of piperaceae extracts and fractions in the control of phytomonas serpens |
description |
ABSTRACT: Protozoa of the genus Phytomonas are harmful parasites to several agricultural crops of economic importance. Due to their recognized biological activity, crude extracts of Piper aduncum, P. crassinervium, P. hispidum, and P. amalago leaves, were tested using the microdilution plate technique to assess the antiparasitic potential against Phytomonas serpens. Results showed that the ethanolic crude extract of P. crassinervium and P. amalago presented the best inhibitory concentration for 50% of the cells (IC50), 16.5 µg mL-1 in chloroform phase, and 18 µg mL-1 in aqueous phase, respectively, after 48 h treatment. Cytotoxicity analyses were performed using the colorimetric method of sulforhodamine-B in LLCMK2 mammalian cells. The chloroform phase of P. crassinervium was subjected to the fractionation process, in which the ethyl acetate and dichloromethane fractions obtained better IC50 values. Scanning electron microscopy (SEM) images showed alterations in the cell membrane of the treated parasites. The data obtained indicate a potential antiparasitic effect of the Piper species analyzed against P. serpens, being considered promising candidates for formulations of bioproducts to control the parasite. |
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Universidade Federal de Santa Maria |
publishDate |
2020 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001000152 |
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